FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8486500
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA95684511.274907205561775Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC8186639.646650562658339Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA4804665.661533023036588Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC3821394.502904613209215Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC3341003.9368408649030817Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG2623983.09194603193307Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC2387722.8135509338360927Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG1409411.6607671006893303No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1354361.5958993695869912Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC1123281.3236080834266188Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA1013911.1947328109350144Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA842280.9924939609968774Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC739610.8715135803923879Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC611140.7201319743121428Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC585650.6900960348789253Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC550240.6483709420844871Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC534100.6293525010310493Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC527850.6219878630766512Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC501100.590467212631827Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA499050.5880516113827844TruSeq Adapter, Index 3 (100% over 40bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG428750.5052141636717139Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC428010.5043421905379131Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA411030.48433394214340425Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGTCTGAACTCCA337570.39777293348258996Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC319540.3765274259117422Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA293300.3456077299239969Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG292750.3449596417840099Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA283580.3341542449773169Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG277280.32673068991928356TruSeq Adapter, Index 3 (100% over 49bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC274440.323384198432805Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAG265480.3128262534613798Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC205150.24173687621516526Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATTTGGTGTTTCTTCGATCAGATCGGAAGAGCACACGTCTGAACTCCA194200.2288340305190597Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC193700.22824485948270784Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA190140.22404996170388264Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA185850.21899487421198374Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG185400.21846462027926708Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACACGTCTGAACTC185240.2182760855476345Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC184610.21753373004183116TruSeq Adapter, Index 3 (100% over 50bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC181280.2136098509397278Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGTCTGAACTCCA174840.20602132799151593Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC168930.19905732634183704Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC162890.19194014022270667Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG160550.18918281977257997No Hit
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA157360.18542390856065516Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTCTCACTACTGCACTTGACTAGTCAGATCGGAAGAGCACACGTCTGAA152750.17999175160549108Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAGATCCGGACTACAACAAAAGATCGGAAGAGCACACGTCTGAACTCCA150610.17747009956990514Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA143860.16951629057915513Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT141530.16677075354975548No Hit
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG133550.1573675838095799Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC132810.1564956106757792Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC124040.1461615506981677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG122300.14411123549166324Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC115630.13625169386672953Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACGTCTGAACTCC115370.13594532492782654Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC115370.13594532492782654Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA112470.1325281329169858Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC111050.13085488717374652Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC101860.12002592352559947Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC100530.11845872856890355Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACGTCTGAACTCC100040.11788134095327873Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAG99220.1169151004536617Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA94970.11190714664467095Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC91690.10804218464620279Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACGTCTGAACTCC88210.1039415542331939Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTGCACGGTATCCATCTGTAAGATCGGAAGAGCACACGTCTGAACTCC86680.10213869086195722Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATAT207.5783563E-444.3264611
CCTAGCG351.3658428E-744.3264611
CAATTAC750.044.3264610
CGATTAC351.3658428E-744.3264610
AGGCGGT207.5783563E-444.3264610
TAGCCAA551.8189894E-1244.3264610
CATTACG1050.044.3264611
TTATACA254.25544E-544.3264610
GGCGGTT207.5783563E-444.3264611
CGTAATC302.4041728E-644.3264610
AATTACT750.044.3264611
GTCCACC600.044.3264611
ATCGCTC407.796189E-944.3264610
GGCAGTT35600.044.32645811
ATACCGA454.474714E-1044.3262029
ATACAAA454.474714E-1044.3262029
CCAATAA1550.044.32629
CAGATAA502.5465852E-1144.32629
CGATGTT254.255589E-544.32629
TAGGTTT302.404271E-644.3261959
Produced by FastQC (version 0.11.5)