FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5424729
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA117923421.73811816221603Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC2429754.479025588190673Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1663713.066899747434388Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA1584832.9214915620669712Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1327482.4470899836655433Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1140832.1030174963578827Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC993721.8318334427397203Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG858801.5831205577273997Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC774661.4280160354554117Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC765631.4113700426325444Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG580531.0701548409146338No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC392200.7229854247096952Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG301750.5562489849723369Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC288890.5325427316276997Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC281150.5182747377795278Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG261930.4828443964666253Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA216700.39946695954765665Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC211060.3890701268210818Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA205620.37904197610608753Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC190570.35129865473464206Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC181190.3340074683915086Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG176430.32523283651588863Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC174390.3214722799977658Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG171710.3165319410425848TruSeq Adapter, Index 16 (97% over 49bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC164240.3027616679100468Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCA160030.2950009115662736TruSeq Adapter, Index 16 (97% over 39bp)
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT157490.2903186500191991Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG156040.28764570543523926Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA154700.2851755359576488Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA153140.28229981626731954Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA152910.28187583195400173Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA149110.274870873733969Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA145820.268806054643467Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTC143830.2651376686282393TruSeq Adapter, Index 16 (98% over 50bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC141840.2614692826130116Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC137470.253413580659974Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC137420.2533214101570788Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA136530.2516807752055448Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC131460.24233468621197482Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT126650.23346788383345968No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA107900.19890394524777183Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC102760.18942881755014856Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC102510.18896796503567276Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA100740.18570512923318383Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA99890.18413823068396598Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC97090.17897668252183657Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA96690.17823931849867525Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC95840.17667241994945737Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACGTCTGAACTCC94320.17387043666144428Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC92470.1704601280543231Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC82720.1524868799897654Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC82680.15241314358744926Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTCAGATCGGAAGAGCACACGTCTGAA82120.1513808339550234Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG78610.14491046465178262No Hit
TCCCCGGCATCTCCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC77720.14326982970024862Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG77070.14207161316261144Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC74600.13751839031959015Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAGAGAAAGGCAGTTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA71320.13147200532966716Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCAGATCGGAAGAGCACACGTCTGAACTC69410.12795109211907177Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGGGTGCCCCCTCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA63890.11777546859944525Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA63240.11657725206180805Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT62450.11512095811606442Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA62020.11432829179116596Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC61720.11377526877379496Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC61490.1133512844604772Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC61340.11307477295179169Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC61320.11303790475063362Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACGTCTGAACTCC60750.11198716101762872Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA60560.11163691310662707Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCCGGCACCTCCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC59530.10973820074698662Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAACGGAATCCCAAAAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCA59280.10927734823251078Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCAATATTACTGTGCTGCTAGATCGGAAGAGCACACGTCTGAACTCCAG57090.10524028020570243Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA57040.10514810970280727Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA56540.10422640467385559Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC56510.1041711023721185Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTAGATCGGAAGAGCACACGTCTGAA55860.10297288583448132Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCA55720.10271480842637484Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTC54950.10129538268178927No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAG54740.10090826656962955Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)