FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8034087
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA180477722.46399622010566Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC5018096.245999078675648Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA2662953.3145645547527676Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA2498923.1103969872370065Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1985442.4712702264737736Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG1842142.2929052174814637Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC1487321.8512620040086696Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC1442151.7950390629327264Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1303681.6226859380536955Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG1196441.4892046849878524No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC933931.1624594057793998Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC739920.9209758370801809Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC685910.8537497789107834Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG575360.7161485804174139Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG415200.5167979883712984Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA360920.4492358621458792Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC358490.446211249641683Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC355820.4428879099765785Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC344620.4289473091341928Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC329410.41001547531163157Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA326390.4062564918702025Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA304110.3785246537658853Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG301620.37542535947146205TruSeq Adapter, Index 11 (100% over 49bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA288600.3592194109921887Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA286680.35682959370492257Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC259710.3232601289978563Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA258050.3211939328015741Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG253690.315767056045074Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC239660.2983039640969783Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC227810.2835543105271327Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC221160.27527707877696617Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC214410.26687537737642125Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG192990.2402139782653586No Hit
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG191700.23860831977547667Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC181780.22626093045793505Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA175970.21902924377094746TruSeq Adapter, Index 11 (100% over 40bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA175800.2188176453653041Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA172170.21429939705656662Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG168540.2097811487478291Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC167930.2090218838805206Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACGTCTGAACTCC166830.20765271772635768Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA162060.20171551540330593Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT161150.20058284158486211No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA158500.19728439584983334Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC156690.19503149517798352Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA155250.19323913221253392Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC146600.18247250745479854Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGAGATCGGAAGAGCACACGTCTGAACT144550.17992087962204042Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC141480.1760996613554222Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTC140930.17541507827834077TruSeq Adapter, Index 11 (100% over 50bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC140320.17465581341103228Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC140290.17461847251591872Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGAGATCGGAAGAGCACACGTCTGAACTCCAG138280.17211663254331203Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGTCTGAACTCCA127400.15857433458213735Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA120450.14992369388083548Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT120230.1496498606500029Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC119490.14872878523720245Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC115170.14335169634085368Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC111740.13908238733287306Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA111440.1387089783817377Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC106250.13224900352709648Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAG103180.12842778526047827Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGTCTGAACTCCA101540.12638648299427177Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAG96870.12057375032159846Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACACGTCTGAACTC96490.12010076565016038Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA89720.11167417031953975Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACGTCTGAACTCC88400.11003117093454427Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC87670.10912254248678165Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAG87630.10907275462663024Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAATTTGGTGTTTCTTCGATCAGATCGGAAGAGCACACGTCTGAACTCCA83960.10450471845774138Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACGTCTGAACTCC83680.10415620343668174Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGAGAAAGGCAGTTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA83650.10411886254156819Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA83530.10396949896111407Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA82550.10274969638740532Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACGTCTGAACTCC81490.1014303180933938Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)