FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4142759
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC3739379.026279346686593Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA2351055.675082716614701Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA2239045.404707345998163Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG1888664.558942482533983Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1200562.897972100235616Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC1013412.446220019074245Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG870842.102077383695262No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA837472.0215271996271085TruSeq Adapter, Index 7 (100% over 40bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA732371.7678315344918687Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC574861.3876259758291516Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC510741.232849895444075Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG422921.020865563263516Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA421291.0169309872961474Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC390570.9427775064878262Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC374560.9041317633972916Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC363660.8778207952719431Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC357850.8637963251060464TruSeq Adapter, Index 7 (100% over 50bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA314920.7601697322967617Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG253640.6122489867259959TruSeq Adapter, Index 7 (100% over 49bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC252420.6093040893761862Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC240870.5814241185644639Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG219910.5308298165546198Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGTCTGAACTCCA185270.44721404262232Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC181380.4378241650069435Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA177270.4279032403284864TruSeq Adapter, Index 7 (100% over 40bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAA172470.4163167589521862Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC148440.3583119365620834Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC146180.3528566349140754Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA137360.3315664753851238Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG131210.31672129612173916No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG121440.29313797882039483Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCC120700.2913517296082152Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT117210.28292739210753026No Hit
TAATTTGGTGTTTCTTCGATCAGATCGGAAGAGCACACGTCTGAACTCCA116400.28097217337527963Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACGTCTGAACTCC101370.24469200356573964Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGTCTGAACTCCA100340.24220573777040855Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA97360.23501246391595554Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGTCTGAACTCCA94370.22779505155863522Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC93600.2259363868378537Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC91560.22101213225292612Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTCCGGTTTTGGATACGTGAGATCGGAAGAGCACACGTCTGAACTCCA90930.21949140657228672Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGTCTGAACTCCA85720.20691524657842755Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC85040.20527382838345173Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC83160.2007357898444008Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCGGAAGCGCACACGTCTGAACTCCAGTCACCAGATCA79620.19219075982937942TruSeq Adapter, Index 7 (97% over 40bp)
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC76640.18499748597492635Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATGGATCTGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA76190.18391125334589822Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC76160.18383883783729635Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC71590.17280754202694387Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC66440.16037621305028846Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC66390.16025552053595202Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAGATCCGGACTACAACAAAAGATCGGAAGAGCACACGTCTGAACTCCA64290.15518643493382067Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC63970.15441400284206733Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAG63180.15250706111555126Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAAGAATTCTCCTTTTGGGAGATCGGAAGAGCACACGTCTGAACTCCAG63110.15233809159548023Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG63010.1520967065668073Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC58670.14162059632240254Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA58540.14130679578512773TruSeq Adapter, Index 7 (100% over 35bp)
GCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA56500.13638254120020016Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGTTCTCAGGTCACCCCTTAGATCGGAAGAGCACACGTCTGAACTCCA55820.1347411230052243Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTCTGATACCAAATTGCTGAGATCGGAAGAGCACACGTCTGAACTCC55540.13406524492494012Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATTGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA55380.13367902887906344Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA55380.13367902887906344TruSeq Adapter, Index 7 (100% over 40bp)
CGCGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA53900.13010653045470422TruSeq Adapter, Index 7 (100% over 40bp)
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACGTCTGAACTCC53780.12981686842029672Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA53780.12981686842029672Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCAGGACGGTGGCCATGGAGATCGGAAGAGCACACGTCTGAACTCCAGT53540.12923754435148171Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCA53020.12798234220238253Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTATGTCTTGTTGATCTCAATAGATCGGAAGAGCACACGTCTGAACTCCA52680.1271616331048946Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG51640.12465122880669621Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAAC50320.12146494642821365Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTC49970.12062009882785843No Hit
TCATGGTCAGATCCGTCATCCAGATCGGAAGAGCACACGTCTGAACTCCA49270.11893040362714799Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATGGATATGTCTTCAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA48430.11690276938629547Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA48200.11634758382034775Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC46270.11168885276696036Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC46220.11156816025262391Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG46130.11135091372681828Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGAATTGAAGTGCTTGAATCAGATCGGAAGAGCACACGTCTGAACTCCA45950.11091642067520703Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCAACCTTTCATCGTTCCCAGATCGGAAGAGCACACGTCTGAACTCCA45910.11081986666373787Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC45660.11021640409205556Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCAAATGTAGACAAAGCAAGATCGGAAGAGCACACGTCTGAACTCCAG45600.11007157307485181Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCCCACTGCTAAATTTGACTGGAGATCGGAAGAGCACACGTCTGAACT44840.10823704685693761Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA44560.10756116877665342Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC44340.107030121713573Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGAAGAGCACACG43560.10514731848992422No Hit
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG43370.10468868693544568Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATGTA3100.044.0213171
TCATGCT1050.044.0213171
TTCTATG254.4321656E-544.0213131
TGTCATG302.5209047E-644.0213131
CTATACG1250.044.0213131
GAATCGT302.5209047E-644.0213131
CGACATG600.044.0213131
GGTGCTC302.5209047E-644.0213131
AATTACA1700.044.0213131
AGCTACA254.4321656E-544.0213131
GGTTCGT4350.044.0213131
TAACTTG254.4321656E-544.0213131
TGATATA454.783942E-1044.021311
TCACATG207.840141E-444.021311
TAACGGA900.044.021311
ATCACAT351.4418038E-744.021311
GGAACGC207.840141E-444.021311
AGATTTC207.840141E-444.021311
TATTACA351.4418038E-744.021311
AATAGCG207.840141E-444.021311