FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197837
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC33605315.290169380167864Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA25262611.494300987743859Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG1784888.121075402770996Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG972274.423758449784947No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC902304.105399990991143Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG459082.088780924154066Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC449922.047103584114746Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC440172.0027417865838095Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC420651.9139271929628994Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC276001.2557801147218832Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA247971.1282456342303822Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC245591.1174168057048817Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA208750.9497974599572215Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG175440.7982393598797364TruSeq Adapter, Index 11 (100% over 49bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG166600.7580179967850209No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG133000.6051404176014873Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC127640.5807528037793522Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG109210.4968976316260032No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAA103710.47187302789060337Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA103670.4716910307725277Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACGTCTGAACTCC101260.4607257044084707Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT89170.40571707547010993No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC83160.37837200847924574Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC81140.36918115401642615Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC75790.34483903947380995Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC72390.3293692844373809Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA69890.31799446455765373Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG68820.3131260416491305No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA63630.28951191557881684TruSeq Adapter, Index 11 (100% over 40bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC58380.26562479383138965No Hit
TAAGATCCGGACTACAACAAAAGATCGGAAGAGCACACGTCTGAACTCCA55180.25106502438533884Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC54950.25001854095640397Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGTCTGAACTCCA54890.24974554527929052Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA52920.24078218721406544Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC50980.23195532698739715Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC50770.23099984211750008Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCC50700.2306813471608677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAG48760.2218544869341994Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGTCTGAACTCCA47080.21421060797502273Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC46260.2104796670544722Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA43660.1986498543795559Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC42550.193599434352957Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACACGTCTGAACT42460.19318994083728686Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACGTCTGAACTCC39990.18195161879611635Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA38700.17608221173817712Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACGTCTGAACTCC37870.1723057715381077Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAG37800.17198727658147533Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGT37610.17112279027061608Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC37080.16871132845611392No Hit
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA36530.1662088680825739Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAGATCCGGACTACAACAAAGATCGGAAGAGCACACGTCTGAACTCCAG35980.1637064077090339Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC35450.16129494589453175Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAAC35270.16047595886319138Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGAAGAGCACACG34150.1553800395570736No Hit
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC33110.1506481144871071Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC32830.14937413466057764Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG32190.1464621807713675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCAGATCGGAAGAGCACA31120.14159375786284423No Hit
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGTCTGAACTCC29730.13526935800971593Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC29020.1320389091638734Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGTCTGAACTCCA27840.12666999418064215Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTAGACTGAAGCTCCTTGAGGAAGATCGGAAGAGCACACGTCTGAACTCC26040.11848012386723855Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA25820.11747913971782258No Hit
GCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA25820.11747913971782258Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAA25590.11643265628888766Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTCTGATACCCAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC25290.1150676779033204Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGTCTGAACTCCA23880.10865227949115426Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCACACGTCTGA23710.1078787917393328Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCA23510.10696880614895464Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC23330.10614981911761427Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGTCTGAACTCCA22920.10428434865733902Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC22540.10255537603562047Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGGCAATGGTAGAACTCACAAGATCGGAAGAGCACACGTCTGAACTCC22400.10191838612235574Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGTCTGAACTCCA22040.10028041205967504Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATATC1300.044.0217171
TACCACA5100.044.0217171
TAATTTG650.044.0217171
TGATGTA1150.044.0217171
TCAGTGC8900.044.0217171
CTAGAAC207.8389817E-444.0217131
AAATCGT254.431245E-544.0217131
TTAGACT302.5202044E-644.0217131
CTATACG454.783942E-1044.0217131
TATGGAT900.044.0217131
TAGCGCT254.431245E-544.0217131
GGCCGGT600.044.0217131
TCGGGTG302.5202044E-644.0217131
GCCGGTA1200.044.0217131
TTCAAGT700.044.0217131
GTCTACG454.783942E-1044.0217131
TCCTATA1050.044.0217131
TATTGCG351.4412944E-744.0217131
AAGCCGT207.8389817E-444.0217131
TAACCGT207.8389817E-444.0217131
Produced by FastQC (version 0.11.5)