FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2094426
Sequences flagged as poor quality0
Sequence length40
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG1697998.107185453198156No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT1383566.605914938030755No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA885464.227697708107138No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC772843.689984749998329No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG752023.590578039042678No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT572962.735642128201235No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC566852.706469457502915No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC485272.3169593960349997No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC436482.0840077424554506No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG400141.9104995831793532TruSeq Adapter, Index 1 (100% over 39bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG377811.803883259661597No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT242171.1562595193145997Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA190630.9101777766318792No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA174980.8354556331901914No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG173890.8302513433274797No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA163800.7820758527634778No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT150640.7192424081824805Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG145910.6966586549250248No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG142560.6806638191084335No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC141830.6771783772737733No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC139330.6652419326345261No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC116430.5559040997390216No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT113390.5413893830576969No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG107310.5123599496950477No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG85290.40722374531255817No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT81790.39051272281761207No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA81480.38903260368234543No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC77850.3717008860661585No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT77730.3711279367234746No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA76480.365159714403851No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG75980.3627724254760015No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG71980.343674114053206No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG71600.3418597744680404No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG70510.3366554846053286No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT66110.3156473420402535No Hit
AGATCGGAAGAGCCCACGTCTGAACTCCAGTCACATCACG65930.3147879180262277TruSeq Adapter, Index 1 (97% over 39bp)
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT64620.30853322103526215No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT64020.30566847432184285No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA63000.30079840490903No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT62590.2988408279881934No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC62410.29798140397416767No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAA60780.29019884206937846No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG57640.27520666760248397No Hit
CGCGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGT57520.2746337182598001Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT57080.27253290400329255No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC52870.25243193123080027No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG51290.24488809821879598No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC50770.24240531773383256No Hit
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGT50480.24102069015567987No Hit
TATTGCACTTGTCCCGGCCTGGAGATCGGAAGAGCACACG50160.23949282524185622No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA44410.21203900257158761No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGC43770.20898327274394035No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTC42440.20263308419586082TruSeq Adapter, Index 1 (100% over 40bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT41950.20029354104656835No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC41560.1984314556828458No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT40640.19403884405560282No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACG39690.18950299509268886No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG39550.18883455419289105No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAG39060.18649501104359859No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG36600.17474954951857932No Hit
TATTGCACTTGTCCCGGCCTGTGAGATCGGAAGAGCACAC35590.16992722588432344No Hit
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT35080.167492191177917No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT33340.15918442570900096No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG33060.15784754390940525No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAG32250.15398013584628914No Hit
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACG32170.15359816961783324No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG32070.15312071183226336No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT30750.1468182690627408No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG30020.14333282722808063No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG29630.14147074186435807No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC29480.14075455518600324No Hit
TCGCTCTGATACCCAATTGATGAGATCGGAAGAGCACACG29220.13951316494352153No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT29120.13903570715795163No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG28190.13459534975215165No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC27910.13325846795255597No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGA27840.13292424750265705No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG27020.12900909366098395No Hit
CCCCCCACTGCTAAATTTGACTGGTAGATCGGAAGAGCAC26250.1253326687120958No Hit
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGT25590.12218144732733455No Hit
TATTGCACTTGTCCCGGCCTGTCAGATCGGAAGAGCACAC25580.12213370154877756No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCA25010.11941219217102919No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGTAGATC24040.11478085165100128No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG23520.11229807116603786No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT22790.10881262933137767No Hit
CTTCTCACTACTGCACTTGACTAGTCAGATCGGAAGAGCA22730.10852615466003573No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACG22640.10809644265302283No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG22340.10666406929631317Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA22330.10661632351775618No Hit
TCCCCGGCATCTCCACCAAGATCGGAAGAGCACACGTCTG22200.10599562839651532Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGT21820.10418128881134975No Hit
TGGAGGCGTGGGTAGATCGGAAGAGCACACGTCTGAACTC20990.10021838919111967Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGT900.034.0021741
TGTTTGT4100.034.0021741
TCTTGTA950.034.0021741
AAATCGT301.4836169E-534.0021741
CATATAT301.4836169E-534.0021741
CGAAACT301.4836169E-534.0021741
TTATGTC1250.034.0021741
CTATACG1150.034.0021741
CTATACA200.002788633634.0021741
GTCTACG1050.034.0021741
TCCTATA553.274181E-1134.0021741
CCTCGCT455.986294E-934.0021741
TATTGCG1550.034.0021741
AAGCCGT301.4836169E-534.0021741
TCGCTCT28800.034.0021741
ATCCCCT455.986294E-934.0021741
TCATGTA200.002788633634.0021741
AGACCGT553.274181E-1134.0021741
TTTGGGG200.002788633634.0021741
TCGTATC504.456524E-1034.0021741
Produced by FastQC (version 0.11.5)