FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3341494
Sequences flagged as poor quality0
Sequence length40
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT47340414.16743528493542No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA2255816.750902440644814No Hit
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG1378074.12411334570704No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1222963.6599197843838716No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT1175563.518067068203624No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT838082.5080996703869585No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC665291.990995644463225No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG637391.90750005835713No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC570091.7060931427678758No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC444641.3306622726241617No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA429291.2847247368991237No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG403331.2070349370670725No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT341571.0222074317655516No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC336801.0079323799474127No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGC330830.9900661201247107TruSeq Adapter, Index 22 (97% over 37bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG319090.9549321351467337No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG283910.8496498871462884No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT272600.81580275170328No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG262450.7854271173313493No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA259170.7756111487855433No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT221140.6617997817742602No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG200690.6005996120298286No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG198480.593985803954758No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGTAGATC168170.5032778751061651No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT167910.5024997800385097No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG153480.45931550378363695No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA149510.4474345906352069No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGC143540.4295683308125048No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG139230.41666990872944853No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG137820.41245023932408675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT136750.4092480788533512No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG131610.3938657379004721No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC129360.387132222891916No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT126490.3785432504143356No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG125200.37468270180943014No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACG121590.3638791510623691No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT115600.34595303777292435No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG112440.3364961900275745No Hit
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCA108700.32530359174668577No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC107220.32087443520772446No Hit
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGT107110.3205452411406395No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC106000.3172233737364185No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT82190.24596782157920977No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG69680.2085294781316381No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT69000.2064944602623856No Hit
TCGCTCTGATACCAAATTGCTGAGATCGGAAGAGCACACG68670.20550687806113074No Hit
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGT67170.2010178680554267No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT66930.20029962645451405No Hit
GCGGGTGATGCGAACTGGAGTCTGAGTAGATCGGAAGAGC64340.19254860251133177No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC62570.187251570704601No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC61570.18425889736746495No Hit
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACG61300.18345087556643824No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC60970.18246329336518335No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGG58820.17602904569034092No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG58050.17372468722074616No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCAGATCG57640.17249769115252042No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG57570.17228820401892087No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA55670.16660212467836244No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACAC54690.16366930480796912No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT53320.15956934233609277No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA51000.1526263401939372No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA50780.15196795205976726No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG48820.1461023123189807No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG48220.14430670831669906No Hit
TTGAATTGAAGTGCTTGAATCAGATCGGAAGAGCACACGT47730.14284029838150242No Hit
TAAGATCCGGACTACAACAAAGATCGGAAGAGCACACGTC46560.13933887057705327No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC45300.13556810217226187No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA42920.12844553962987812No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT42810.12811634556279317No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAA42040.12581198709319844No Hit
GATGGATATGTCTTCAAGGCCAGATCGGAAGAGCACACGT41660.12467477122508676No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGAAGATC41280.12353755535697505No Hit
TCGCTCTGATACCCAATTGATGAGATCGGAAGAGCACACG39130.11710330768213262No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTAGATCGGAAGA38020.11378144027791161No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA37890.11339239274408394No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGA37670.11273400460991401No Hit
CGCGACCTCAGATCAGACGTGGTAGATCGGAAGAGCACAC37620.1125843709430572No Hit
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACG37280.11156686200843097No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT37180.11126759467471735No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG37080.11096832734100376No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC36140.1081552144040959No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTG34250.10249906179690882Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCCG504.456524E-1034.005541
TTATGTC4850.034.005541
TATGGAT800.034.005541
GTCTACG1300.034.005541
TTTCCGG1550.034.005541
TCCTATA351.0907861E-634.005541
ATTCCGG200.002787517234.005541
CTACGGG408.061943E-834.005541
AAACCGC2400.034.005541
ATTGGTT252.0270066E-434.005541
AGCTACA252.0270066E-434.005541
TAATGCA252.0270066E-434.005541
AAGCTAT200.002787517234.005541
TCGCCCT455.982656E-934.0055351
CGAAACT750.034.0055351
CTATACG900.034.0055351
TATTGCG455.982656E-934.0055351
AAGCCGT3000.034.0055351
TCGAAAC455.982656E-934.0055351
TATCAGG1700.034.00299523
Produced by FastQC (version 0.11.5)