FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8197047
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA264372032.252102494959466Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA3693704.506134953233768Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC2436852.9728388772200525Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1890612.3064525554141633Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1639572.0001959242151472Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC1434761.7503376520837322Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1114661.359831168468352Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC978421.1936249725053425Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC893541.090075486940602Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG782600.9547340645966773Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC763070.930908411285186Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG686750.8378017107868236No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG613100.7479522808640722Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA401080.489298158226981Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC389930.47569569870710754Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA385950.4708402916318523Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA374120.45640826507399557Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC346910.4232133840393986Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC345560.4215664494786964Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC338640.4131243849156897Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC311390.37988070582003497Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC308780.37669663233601075Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC305250.3723902034476562TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG284000.3464662335106777TruSeq Adapter, Index 12 (100% over 49bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG263710.32171341703908735Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA262680.32045686696684794Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC260890.31827315373450954Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA241370.29445969993828264Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC227430.27745357565962475Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA221090.2697190829819568Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA220060.26846253290971733Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG213170.2600570668925041Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA211300.25777575753805Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC209770.25590923170258756Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC203610.2483943302996799Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG198960.24272155570170573Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC171980.2098072635181914Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC171240.2089044993886213Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA170270.20772114640796863Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA168630.20572042590459713TruSeq Adapter, Index 12 (100% over 40bp)
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT159480.19455786943761577Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC153080.18675017966836105Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA151140.18438347370705574Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT151010.18422488000861773No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC149100.1818947725931058Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC135940.16584021050507577Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA134700.1643274706122827Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC126270.15404327924434252Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA121720.148492499799013Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA121300.14798012015790563Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC119270.14550361855922017Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA113870.1389158803164115Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA106400.12980284241385953Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC106360.1297540443528017Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG106020.12933926083381003No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC105730.12898547489114068Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGAAGGTAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC104250.12717994663200052Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACGTCTGAACTCC103790.12661876892983534Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATTTGGTGTTTCTTCGATCAGATCGGAAGAGCACACGTCTGAACTCCA103620.1264113771703395Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC98490.12015302583967127Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA94070.11476084009277976Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACCGAGTAGATCGGAAGAGCACACGTCTGAACTCCA89380.1090392674337478Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG85840.10472063903012878Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC84700.1033298942899803Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTCTCACTACTGCACTTGACTAGTCAGATCGGAAGAGCACACGTCTGAA83310.10163416166822027Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACGTCTGAACTCC82560.10071919802338573Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATGTA3050.044.0215841
AATTACA3450.044.0215841
AATCCGT10200.044.021581
CAACGGG900.044.021581
CGAAACG254.4323893E-544.021581
TTTAAAC650.044.021581
ATAGCGT254.4323893E-544.021581
TAGTTGC254.4323893E-544.021581
TATTGCG2000.044.021581
ATATAAT254.4323893E-544.021581
TCTAAGT1950.044.021581
AAACCGA1850.044.021581
TATTACA1150.044.021581
GTCAAGT1750.044.021581
TTACGTC254.4323893E-544.021581
TTACATG254.4323893E-544.021581
TATTTCA351.4419675E-744.0215761
ATTTACA207.840358E-444.0215761
TTAGGTA408.287316E-944.0215761
CAAACCG408.287316E-944.0215761
Produced by FastQC (version 0.11.5)