FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6692132
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG105541215.770938170376795No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT4791527.159930497485704No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC3411055.097105078022968No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC2730944.0808220758347264No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG2414813.608431513305476No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT2096183.132305220518663No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC1686402.519974202541133No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1384752.0692209896636826No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA1234371.8445093432108033No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1210781.8092589925004467No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT715511.0691809426353216No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA621950.9293749734763151No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC602020.8995937318630296No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG580880.8680043968050839No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG457140.6831006919767871No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG420210.6279164846120787No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG406680.6076987124581523No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC357820.5346876003043575No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC323170.4829103789345458No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT310140.46343975283213185No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT297770.4449553595177142No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT285140.4260824502565102No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG285050.42594796396723794No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT281400.42049379779119717No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT266220.39781044366727975No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG265620.3969138684054648No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG261070.39011483933670166No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC247810.37030052605059194No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG218350.3262786806954794No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC212420.31741752852454197No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACTAGATCGGA180030.26901740730756657No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC175120.2616804330817145No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA171920.2568986983520349No Hit
TATTGCACTTGTCCCGGCCTGGAGATCGGAAGAGCACACG170130.25422391548762036No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG159900.238937307273676No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA159340.2381005036959821No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT152170.22738642931729383Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT143540.21449068846818922No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAG142100.21233890783983342No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC141470.21139750381492772No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG140650.21017218429044737No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT132740.19835233375552064No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTC128770.19241999410651195TruSeq Adapter, Index 21 (97% over 40bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGC127750.1908958161614266No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG123640.1847542756179944No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT122450.1829760680153948No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC119510.17858284923250167No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT118150.1765506119723879No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG116930.17472757560669752No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC112470.16806303282720664No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG111050.16594113804091132No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT109710.16393878662285802No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG109650.1638491290966765No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG107230.16023294220735634No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG103720.15498797692573904No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCG103280.1543304884004081TruSeq Adapter, Index 21 (100% over 39bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG100750.1505499293797552No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT100440.1500866988278175No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT100410.15004187006472675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG95330.14245086618136044No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAG90300.13493457690314536No Hit
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGT90300.13493457690314536No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACAGATCGGAA89600.13388857243102797No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG86130.12870337883353167No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC85140.12722402965153706No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT84430.1261630822583894No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT84380.12608836765323816No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA83040.12408601623518484No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT82330.12302506884203719No Hit
TATTGCACTTGTCCCGGCCTGTGAGATCGGAAGAGCACAC81950.12245723784288774No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA81050.12111237495016536No Hit
CCCCCCACTGCTAAATTTGACTGGAGATCGGAAGAGCACA77960.11649501235181851No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG77560.11589729551060857Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC75780.11323745556722432No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA75780.11323745556722432No Hit
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT74040.11063738730796105No Hit
TGGAGAGAAAGGCAGTTCCTGAGATCGGAAGAGCACACGT73030.10912815228390593No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG71220.10642348357743092No Hit
TGAGGTAGTAGTTTGTGCTGTAGATCGGAAGAGCACACGT69000.10310615510871572No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT68040.10167163468981184No Hit
ATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACG67820.10134289042714639No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAAT252.0279248E-434.0035362
CGAACAA1750.034.00353627
CACATTG1200.034.0035363
AGCTTAT1200.034.0035362
ATTACAC1100.034.0035362
TAAGTCT252.0279248E-434.0035363
ATCGTTA900.034.0035363
TCAATCA351.0915064E-634.00353627
AGTGCAT2100.034.0035363
CGCTCAT504.456524E-1034.0035363
GAGGTAC301.4836949E-534.0035362
TGATTCC455.988113E-934.0035362
CCATATG601.8189894E-1234.0035363
TTAACAT252.0279248E-434.0035363
CATGTTC301.4836949E-534.0035362
CATGTAC1850.034.0035362
CATAACG301.4836949E-534.0035363
CATAAAG6750.034.0035363
GGTCTTC301.4836949E-534.0035363
ATCGGCG252.0279248E-434.00353623
Produced by FastQC (version 0.11.5)