FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6346266
Sequences flagged as poor quality0
Sequence length40
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG94654914.91505398607622No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT63832510.058276788272034No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT3866026.091802644263572No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC3792955.9766640730155345No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC3117634.9125422728892865No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC2172083.4226110282802513No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC2078913.275800289493066No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1749992.7575112672554223No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC1219061.920909082600698No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT1157021.8231508102559837No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA1008481.58909191641195No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG899471.4173216187282411No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG638471.006056159637809No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC465900.734132480422346No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC418700.6597580372458387No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT392860.6190411810661577No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT353420.5568944005813812No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG328140.517059953049557No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG301000.47429464822306533No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT271780.42825182556167674No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG229730.361992390485996No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT223650.35241195373783574No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC211700.33358198348446155No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG207340.32671180186900456No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC198900.31341264296201893No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA190300.29986136729850277No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG181610.2861682759594382No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA178060.28057443542391697TruSeq Adapter, Index 12 (100% over 39bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT176890.27873083164178747No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT173590.27353092353834524No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT172570.27192367921546307No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG163490.2576160532823553No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG156310.24630231383304763Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC147770.23284558195323046No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC145540.22933170465908614No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC138670.2185064414255564No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC137180.2161586041303658No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT136930.21576467169828686No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG130020.2048763792756244No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA129100.20342670792557388No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC121320.19116753063927672No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC110130.17353511497942253No Hit
CCCCCCACTGCTAAATTTGACTGGTAGATCGGAAGAGCAC107930.1700685095771277No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT105490.16622372904003707No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG103820.16359226039374966No Hit
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGT102770.16193774417901802No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC98700.15552452418477258No Hit
CTAGACTGAAGCTCCTTGAGGAAGATCGGAAGAGCACACG96380.15186883121507985No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT94940.14959978040630506No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA91720.14452593068112807No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA90570.1427138414935649No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT88620.139641168523349No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA84970.13388975501499623Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC84200.13267644312419302No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG80700.12716138907508762No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA77640.12233965610644118No Hit
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG76950.12125240259390326No Hit
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT75040.11824275881282No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG74270.11702944692201682No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG73590.11595795070676206No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC70980.11184529611585772No Hit
CTTCTCACTACTGCACTTGACTAGTCAGATCGGAAGAGCA68980.10869383665922606No Hit
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG68300.10762234044397131No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG66680.10506965828409967No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA66360.10456542477103858No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT65850.10376180260959751No Hit
TCCTGTACTGAGCTGCCCCGAGTTAGATCGGAAGAGCACA63740.1004370128828511No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)