FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7658344
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG115129615.033223892789355No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC5495767.176172812294668No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT5216246.811185290188061No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC3443324.496167839940332No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC2581353.3706373074910188No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT2296632.9988598057230127No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1988582.596618799051074No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1624022.1205889941741973No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1215011.586517920845551No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT805471.0517547918975694No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA735220.9600247782027027No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG619730.8092219414536614No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC558150.7288129130788589No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT545500.7122949817871853Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA487240.6362210942731222No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG485790.6343277345598474No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC484400.6325127207657426No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT389030.5079818822450389No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG361350.4718382982012822No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG356400.46537475986975774No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG345610.45128555207235405No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG330850.43201245595653576No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC321030.4191898405190469No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT313750.40968386899308784No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA271100.3539929781164179No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC243160.31750989508959115No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG229930.30023461991260775No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT210730.27516392577821003No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG196910.2571182490627216No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT191050.24946646429045236No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT182810.23870695805777334No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT181050.23640881109545353No Hit
GGCTGGTCCGATGGTCGTGGGTTATCAGAACTAGATCGGA179010.23374504984367378No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC177970.2323870539113939TruSeq Adapter, Index 2 (100% over 40bp)
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC177390.23162971002608396No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG173490.22653722528003442No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC165730.21640448640071536No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA156630.20452202199326644Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT149010.19457209025867733No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG145010.1893490289806778No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT143710.18765153406532797No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG141880.1852619835306432No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC141340.18455687025811324No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG140980.18408679474309328No Hit
AAACCGTTACCCTTACTGAGTAGATCGGAAGAGCACACGT140580.18356448861529334No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT137770.17989528806749866No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG136380.17808027427339385No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT133510.17433272780642917No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT131210.17132946757157944TruSeq Adapter, Index 2 (100% over 39bp)
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC130780.1707679884841945No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT129600.16922718540718465No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT124580.16267224350329523No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC124430.16247637870537024No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC122760.16029575062180543No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT121540.1587027169320156Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG121360.1584676791745056No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT120440.1572663750805657No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG113590.14832188264199153Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT111540.14564506373701677No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACG110470.1442478948451519No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA107320.14013473408872726No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG101950.1331227743230129No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG101550.13260046819521296No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG99910.13045901307123317No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC96440.12592800741256857No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC95880.12519677883364863No Hit
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACG93320.12185401961572893No Hit
TCCTGTACTGAGCTGCCCCGGGAGATCGGAAGAGCACACG90060.11759722467415933No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG89390.11672236191009441No Hit
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTC89000.11621311343548944No Hit
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGT87270.11395413943275465No Hit
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACA86880.1134448909581497No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT86590.11306621901549473No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG84470.11029799653815499No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT80860.1055841837347604No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT80670.10533608832405544Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC79890.10431759137484553No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)