FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6853213
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG121393117.71331198957336No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT6440109.397198073370841No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC4927177.189576626321113No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC3008524.389940893417438No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC2731813.986174076305523No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1283881.8733986525736177No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT1175441.7151663022877006No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG972741.419392626495047No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT924971.3496880952043953No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC649250.94736585598609No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT553190.8071980252182442Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA532260.7766576057099057No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG504730.7364866669108344No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC449100.6553130626466739No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC444120.6480463980909392No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT394780.576050970544765No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG350390.5112784324666401No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG310220.4526635900562262No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA296970.4333295929952856No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC235010.34291944522955875No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG229420.3347626872242261No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCA206730.3016541292383587No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC200400.2924175857367924No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT198360.28944087977420224No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG193650.2825681910076339No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG179640.2621252250586696No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC174690.25490233559062003TruSeq Adapter, Index 5 (100% over 40bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA174420.2545083598014537Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT174290.25431866775481804No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC173410.2530345985160537No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA169020.24662884401812696No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG166980.24365213805553684No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT162510.23712964999044972No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT161800.23609363958190122No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT158370.23108868788989925No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG155800.22733862204487149No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG144860.21137530673568736No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT142540.20799003328803584No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG135390.19755697072307543TruSeq Adapter, Index 5 (100% over 39bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC134290.19595188417462No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG131920.19249365224749326No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT129660.18919592897521206No Hit
GGCTGGTCCGATGGTCGTGGGTTATCAGAACTAGATCGGA127940.18668615728126356No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT126500.1845849530723764Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG126200.18414720219552494No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC125980.18382618488583383No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT123610.1803679529587071No Hit
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT116100.16940958934152492No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT116080.1693804059497348No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC108920.15893275168887938No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT107360.15665644712925164No Hit
TCCTGTACTGAGCTGCCCCGGGAGATCGGAAGAGCACACG104210.15206006292231106No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT97170.1417875090121962No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT92610.1351336956840536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG92050.1343165607139308No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACT90290.13174842223640212Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT89650.13081455369911893No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACAC87290.12737091346788726No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG83600.12198657768261398No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG80650.11768202739357438No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA80550.11753611043462386No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC80300.11717131803724763No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG79460.11594561558206347No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG78720.11486583008582982No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC78550.11461777125561397No Hit
CAACGGAATCCCAAACGCAGCTAGATCGGAAGAGCACACG77840.11358176084706546No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG75870.11070719675574071No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG73100.10666529699281198No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC72420.1056730616719486No Hit
AAACCGTTACCCTTACTGAGTAGATCGGAAGAGCACACGT72330.10554173640889317No Hit
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG70660.10310492319441991No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT69620.10158738682133477No Hit
GGCTGGTCCGATGGTCGTGGGTTATCAGAACAGATCGGAA69500.10141228647059415No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCA200.002788057634.004727
TATCCGA7150.034.004723
CAACAGT252.0283918E-434.00221634
TTATCCG7150.034.0019722
AAACTGT408.0735845E-834.000981
TTCTAAT351.092154E-634.000982
TGATATA351.092154E-634.000981
TTGCTTC351.092154E-634.000982
TGGCGCG200.00278955434.000981
TTGTATT200.00278955434.000981
TATATGA408.0735845E-834.000982
TTCAAGT1750.034.000981
CGACATG351.092154E-634.000981
TAACGGA351.092154E-634.000981
ATCACAT351.092154E-634.000981
TTTCAGT351.092154E-634.000982
TCATGTA1750.034.000981
TTGGTTC200.00278955434.000982
GATATAG408.0735845E-834.000982
TATTACA408.0735845E-834.000981
Produced by FastQC (version 0.11.5)