FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9185832
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA128577613.997382055321717Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA8417899.163992983977936Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC7565058.235563202113864Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC3939764.288952813419622Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC2466592.685211312377583Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA2448882.665931621653869Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC1883582.0505273773785544Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG1798971.9584181378453254Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1735631.889464122574852Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC1342411.4613918477934278Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC1187241.2924686626099846Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA796280.8668566984460416Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG734410.7995029737099482No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG696660.7584070773338768Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC487740.5309698675090073Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC477570.5198984697303413Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC367930.40054074579199794Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGTCTGAACTCCA362850.3950104900677478Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA344240.3747510296291071Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG334250.3638755857934262Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC330140.3594013040952632Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC329340.3585303976819955Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG317850.3460220043214376TruSeq Adapter, Index 16 (97% over 49bp)
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACGTCTGAACTCC284060.3092370946910416Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACGTCTGAACTCC281730.30670057976239934Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA274970.29934142057028695Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC271210.29524816042792856Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC266820.29046906148512186Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA262980.2862887107014367Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC236850.2578427299780793Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG227460.24762046595234924Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG225050.24499686038238017Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA216400.2355801847889228Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA210150.22877622843526854Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC200710.2184995327587093Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA196050.2134265029014247Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC191510.20848410900613032Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA189230.20600202572831727Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA183880.20017783908958925Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC165320.17997281030177778Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC159830.17399621504072793Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT150750.1641114272501391Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC148480.16164023030249192Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG148380.16153136700083345No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG141990.1545750020248574No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG139460.1518207604928982No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA131020.14263269783292357Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA130910.14251294820109925Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGTCTGAACTCCA126240.13742903201364884Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC126130.13730928238182455Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA124810.13587228679993277Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACACGTCTGAACTC122870.1337603387477585Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA116190.1264882701969729Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA115950.12622699827299258Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA109950.11969520017348456Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA109420.1191182246746947Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAG107810.11736552551799337Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT107740.11728932120683244No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC106220.11563459902162374Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAG105320.11465482930669753Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC104790.11407785380790766Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC104430.11368594592193718Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC103950.11316340207397652Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC103090.11222717767971373Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC102780.11188970144457247Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC101540.11053979650400747Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA101320.11030029724035885Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC95600.10407331638549452Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGAGATCGGAAGAGCACACGTCTGAACTCCAG95550.10401888473466529Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC94590.10297379703874401Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGGG900.044.018961
TGCGGGT2850.044.018961
TTCTACG302.5221707E-644.0189551
CAAAACG502.7284841E-1144.0189551
CAACGGT302.5221707E-644.0189551
GCGAATC351.4426769E-744.0189551
GTCTACG1650.044.0189551
AATGGCG2800.044.0189551
TTCCGGT302.5221707E-644.0189551
TTATATG302.5221707E-644.0189551
TATGACA408.290954E-944.0189551
TATTGCG1950.044.0189551
ATATAAT207.8427064E-444.0189551
CTACGGG207.8427064E-444.0189551
TCATGTA2800.044.0189551
CGATGTA254.433992E-544.0189551
TATGGGC600.044.004582
CGTATCC254.442551E-544.0045782
AACGGAC4200.044.0045782
TCTACGG1950.044.0045782
Produced by FastQC (version 0.11.5)