FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6290612
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG75915112.067999107241075No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT5252508.349744031264366No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC3859906.135968964545898No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC2719414.322965714623632No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG2315383.6806911632763235No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT2132203.3894953305020246No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC1970673.1327158629398855No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1529872.4319891291976043No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG964161.5326966597208664No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC739281.175211569240004TruSeq Adapter, Index 9 (100% over 40bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG670911.0665258006693148No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA583480.9275409133483357No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG568390.9035527862789821No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC490550.7798128385600638No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC435950.6930168320665779No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG425490.6763888791742362No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT423920.6738930965699363No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG364880.5800389532846725No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT316290.5027968661872644No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC295430.4696363406294968No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG289660.4604639421410826No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG278180.44221452539117023No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT273200.434297966557149No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT271800.43207242792911094No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG251480.39977032441358645No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA240440.38222036266105747No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG236700.37627499518329854TruSeq Adapter, Index 9 (100% over 39bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG204800.3255645078730019No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT200180.31822023040047615No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT197590.31410298393860564No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT194870.3097790803184174No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC179860.2859181268849517No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT177730.2825321288294366No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA167050.26555444843840315No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT164120.2608967140240091Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT159330.2532821925752216No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC158210.25150176167279115No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT147050.2337610394664303No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG140790.22380970245820278No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG140400.2231897309832493No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC139690.22206106496474431No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC138530.22021704724436986No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC115680.1838930774938909No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT114950.1827326180664139No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC109830.17459350536958884No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG108210.17201823924285906No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG104830.16664515312659564No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG101280.16100182303407046No Hit
TTATGTCTTGTTGATCTCAATAGATCGGAAGAGCACACGT100240.15934856576752784No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC99390.15799734588621903No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA95590.15195659818154417No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC93280.14828445944528132No Hit
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGT91200.14497794491219615No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACTAGATCGGA82340.13089346473761218No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT79840.12691928861611557No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG76020.12084674750246875No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC75690.12032215625443121No Hit
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGT74930.11911400671349623No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG74660.1186847956923746No Hit
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGT74480.11839865501162684No Hit
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACG74360.11820789455779501No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG72590.11539417786377543No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG67820.10781144982395989No Hit
CAAAGAATTCTCCTTTTGGGCTAGATCGGAAGAGCACACG65690.1044254517684448No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG65130.10353523631722954Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)