FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6053872
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA162390226.82418789165017Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA2805784.634686693078413Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC1948373.2183865136230163Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1648752.723463594869531Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG1447832.3915768288460675No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC1288512.1284064149357635Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1040381.7185365002761868Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC808281.3351455068755995Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA735081.2142311565226356Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC711481.1752478413815157Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC578500.9555867715736309Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG486900.8042786500936921Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC469100.7748759801991189Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC463690.7659395507536334Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC455540.7524770923468485Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC420290.6942498949432694Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA409180.6758980037899711Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC384850.6357088488160965Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA342800.5662491707786356Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG291290.48116312997698TruSeq Adapter, Index 2 (100% over 49bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG280700.4636701932250963No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG271020.44768042667568786No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC264240.43648098274955266Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA229890.3797404371945756Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG225820.37301746716811984Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA213320.35236952482642514Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG210980.3485042300200599No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC196780.3250481675198947Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC193100.3189694132944998Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG174080.28755150422737713Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA174070.2875349858735038Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC146130.2413827051513478Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA136460.22540945695581274No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC133000.21969410651563165No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGAAGAGCACACG132490.21885167046809048No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT131390.21703465154202134No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC127530.21065856694690602Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC125510.2073218594644882Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA124160.20509188169158515Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC122620.20254805519508834Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA121080.2000042286985916Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA118340.19547819973729208Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA104870.17322797706988188Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC101670.16794210383040803Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA101470.16761173675294092Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA97690.16136779898881246Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACACGTCTGAACT94010.15528904476341754Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC92350.15254699802044047Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC91090.15046568543239763Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA89400.14767408362780052Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA88020.14539455079327743Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGATCGGAAGAGC85340.14096763195521808No Hit
AATTGCACGGTATCCATCTGTAAGATCGGAAGAGCACACGTCTGAACTCC84370.1393653516295026Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACGTCTGAACTCC83580.13806040167350747Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC82910.13695367196399263Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT81480.13459154736010276No Hit
AAACCGTTACCATTACGGAGTAGATCGGAAGAGCACACGTCTGAACTCCA80350.13272497337241357Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG79210.13084188103085098Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC76410.12621674194631138Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA74040.12230189207832606Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA73680.12170723133888527Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC72730.12013798772091647Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC71960.11886607447266807Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC71260.11770978970153316Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC70800.1169499454233588Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG69340.11453826575784885Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC67010.11068948930535696Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA65040.1074353735923059Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA64920.10723715334582562Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA64160.10598175845145058Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACCGAGTAGATCGGAAGAGCACACGTCTGAACTCCA64130.1059322033898305Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC63570.10500717557292258Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC62700.10357007878594063Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCA61170.10104277064331721Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)