FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7951168
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA158278819.90635841174529Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC4866716.120748549143975Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA3357334.222436250875343Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC2629183.306658845593503Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG2090592.6292866658080927Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA2007192.5243964157215646Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1648612.0734186474238756Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1342011.6878149222856316Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC1100291.383809271795037Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG858921.080243808205285No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC856381.0770493089820263Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC615800.7744774101113195Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC534500.6722282814298478Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG504320.6342715938086078Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC449350.565137096839106Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC403310.5072336542253918Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA399410.5023287144731441Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC393070.4943550431835926Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA365260.4593790497194878Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA361910.4551658322399929Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC333150.4189950457593149Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC325190.40898393795729127Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC313140.3938289317997054Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA301910.37970522066695106Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG294660.37058706343520853Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA258990.3257257298550352Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC251360.3161296554166633Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC244200.3071246891022803Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC219180.27565761407632183Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC217600.27367048463823174Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA211200.2656213527371073Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA203710.2562013530590726Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC189990.23894602654603703Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC187110.235323917190531Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG186850.23499692120704782TruSeq Adapter, Index 3 (100% over 49bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC180830.22742570651255262Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC177140.22278487890081056Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA171770.21603115416502333Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG170050.2138679499665961Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA169780.21352837721451742Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA169610.2133145721483938TruSeq Adapter, Index 3 (100% over 40bp)
GCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA166660.20960442541271923Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG160750.20217155517277463Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA155740.19587059410642563Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC154120.1938331575939535Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA134060.1686041597913665Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC134040.1685790062541755Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG132100.16613911314664714Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTCTCACTACTGCACTTGACTAGTCAGATCGGAAGAGCACACGTCTGAA131560.16545996764248974Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG124020.15597708412147748Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC122350.15387676376602785Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGTCTGAACTCCA119430.1502043473361398Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT117960.14835556235260028No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG115740.14556351972439774No Hit
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA115100.14475860653428527Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC108040.13587940790585734Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGTCTGAACTCCA106620.13409350676529536Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG105590.13279809959995814Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATTGCACGGTATCCATCTGTAAGATCGGAAGAGCACACGTCTGAACTCC104740.13172907426934005Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAG102660.1291131064014746Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC101980.12825788613698014Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAG100890.12688701836006988Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA96250.12105139773175462Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATGGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC95020.11950445519450727Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC93320.11736640453327109Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC91580.11517804679765287Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA89990.11317834059096725Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGAAGGTAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC89110.11207158495456265Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA88290.11104028992973108Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC87750.1103611444255737Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC86210.10842432206186563Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC85760.10785836747506783Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA85420.10743075734282058Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC84230.10593412187995524Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATGGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC81850.10294085095422459Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC81760.10282766003686503Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC81060.10194728623517954Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAAC4350.044.0184361
TAACGGA1850.044.0184361
TAACCGT49850.044.0184361
AATTGCA20600.044.0184361
TGTGTTC3750.044.0184361
TATTTGT207.843102E-444.0184331
TTAGGGG254.4342487E-544.0184331
TACCCGT254.4342487E-544.0184331
TTCAGGT254.4342487E-544.0184331
GGTAACA254.4342487E-544.0184331
CAACGGG302.5223344E-644.0184331
TCACATG408.292773E-944.0184331
TTTAAAC800.044.0184331
GATTTAT207.843102E-444.0184331
TGCGGGT1050.044.0184331
ATCACAT1450.044.0184331
TTCTTGC254.4342487E-544.0184331
GTTAAAC600.044.0184331
TCATGTA2150.044.0184331
AAACCGA700.044.0184331