FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5191233
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG88732017.09266372748054No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC4145307.985193498346153No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT3872157.459017925028601No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC2953005.688436639233878No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC2590834.990779647147412No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA1276082.458144336807845No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG960911.8510246024403068No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG846081.6298247449112764No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC628231.2101749237608868No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT497190.9577493439419884No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC418010.8052229595550806No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA393260.7575464249052201No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG376300.7248759591411135No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC335450.6461855979109395No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT314020.6049044610403733No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT235870.45436219102475267No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG232470.44781268727487283No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC208880.4023706891985006No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACTAGATCGGA184790.3559655288059696No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC183120.3527485666699992No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG173160.33356237333211586No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA171900.3311352042953957No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT161320.3107546896854755No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCA157760.30389697399442483No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT152820.2943809303107759Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG150930.2907401767556956No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG146270.2817635039690956No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG144720.2787777007890033No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG142920.2753103164508316No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA128960.2484188245836779No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC127450.24551007438887834No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC124190.23923025608752294No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG122520.23601329395155252No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC121350.23375949413174096No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC121340.23374023088541776No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG117950.22720999038186113No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT103350.1990856507500241No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT102150.196774061191243No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG101700.1959072151067001No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA99140.190975824047967No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAG90800.17491027661443823No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC90380.17410122026886482TruSeq Adapter, Index 6 (100% over 40bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA87780.16909277622483906Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT87630.16880382752999143TruSeq Adapter, Index 6 (100% over 39bp)
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT82420.15876767619561674No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG78140.1505230067692974No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG78040.15033037430606563No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT77380.149059000048736Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC76740.14782615228405274No Hit
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT76160.14670888399730853No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGC73480.14154633398269736No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT71450.13763589497909262No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG70420.1356517806078055No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG70230.13528577892766516No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG69090.13308976884682308No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACAC69000.13291639962991453No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC67270.12958385801600505No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT65950.1270411095013458No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG65500.1261742634168029No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT64230.12372783113375956No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC63830.1229573012808325No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG63770.12284172180289346No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG63320.12197487571835054No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT62350.12010634082500246No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT62160.11974033914486212No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAG61450.11837264865591662No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT60520.11658116674786125No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG59110.11386504901629343No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT58260.11222767307882348No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA57000.10980050404210329No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCAC56700.109222606652408No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACAGATCGGAA53920.10386742417456507No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC53220.10251899693194276No Hit
TCCTGTACTGAGCTGCCCCGAGATAGATCGGAAGAGCACA53150.10238415420768053No Hit
TCCTGTACTGAGCTGCCCCGGGAGATCGGAAGAGCACACG52980.10205667902018654No Hit
TCCGGGTGCCCCCTCCAAGATCGGAAGAGCACACGTCTGA52890.10188330980327794Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATATA200.0027885334.0033721
CAACGGG601.8189894E-1234.0033721
TATGGAT455.988113E-934.0033721
GTCTACG1600.034.0033721
TTTCCGG3050.034.0033721
AAAGTTC301.4836798E-534.0033721
TATTGCG850.034.0033721
TCATGTA1450.034.0033721
TCGTATC252.0279203E-434.0033721
GAACCGT301.4836798E-534.0033721
CAGAGTC252.0279203E-434.0033721
TATCGCA1000.034.0033721
TCGAAAC1300.034.0033721
GTAAATG455.988113E-934.0033721
TGTTTGT4500.034.003371
GTGTTCT2250.034.0020642
TTCTAAT301.48407125E-534.0020642
GGTTTAC1950.034.0020643
TATAAAG301.48407125E-534.0020642
GTAAACC1350.034.0020642
Produced by FastQC (version 0.11.5)