FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8433128
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG147559417.49758808356757No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT8360959.91441135483773No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC6050867.175107504593788No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT3679104.362675391622183No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC3505754.157117027039077No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC3415924.050596646938123No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG2126242.52129458962321No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1683041.995748196873094No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1256781.490289249730349No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC830610.9849370245536414No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT710920.8430086677209215No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG560110.6641782266319212No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG549840.6520000645074995No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC532080.6309402632095706No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT515880.6117303093229464No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA455320.5399182841764052No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG419760.4977512495956423No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT390700.46329191256198177No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG365330.43320817613583No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG353620.41932246255481953No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT310520.36821449881941787Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC306590.3635543063024776No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT284620.33750228859327164No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC270880.32120940177831997No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT266210.3156717175406326No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA247620.29362770255591997No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG245980.2916829911748049No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT222120.2633898121788262No Hit
AAACCGTTACCCTTACTGAGTAGATCGGAAGAGCACACGT220860.26189570465431095No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG218240.25878890964301743No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC190370.22574067416028784No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC187790.22268131113389955No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA187670.2225390151791838No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG179380.21270873630757176No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG163680.19409168223226306Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG162150.19227740880963742No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT156890.1860401027945977No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT150610.1785932811644742No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA147410.1747987223720546TruSeq Adapter, Index 12 (100% over 39bp)
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC143690.1703875477758668No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC143150.16974721597964598No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG142470.16894087223625684No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG142240.16866813832305166No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG141090.16730446875702587No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC140440.16653369900231563No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG140030.16604752115703686No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT138810.16460084561742688No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT137520.16307116410423272No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT131200.155576910489204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG122870.1456991996326867No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT121280.1438137782327032No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT117800.13968719554594689No Hit
TCCTGTACTGAGCTGCCCCGGGAGATCGGAAGAGCACACG117050.13879784582897353No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC116870.13858440189689994TruSeq Adapter, Index 12 (100% over 40bp)
GGCTGGTCCGATGGTCGTGGGTTATCAGAACTAGATCGGA114460.13572662480635891No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT114280.13551318087428532No Hit
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT110820.1314103141799816No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT105850.1255168900555049No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC104380.12377376461023715No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG102440.12147331334233277No Hit
TCCCCGGCATCTCCACCAAGATCGGAAGAGCACACGTCTG102420.12144959734988015Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT100230.11885269617631795No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT98420.11670639885935563No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC96880.11488026744050368No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG96240.11412135568201977No Hit
CAAAGAATTCTCCTTTTGGGCTAGATCGGAAGAGCACACG90180.10693540996887514No Hit
CAACGGAATCCCAAACGCAGCTAGATCGGAAGAGCACACG85980.1019550515538244No Hit
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG84440.10012892013497245No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAACG252.027546E-434.0047434
TCAATTG504.456524E-1034.0031327
AGAATAT252.0287471E-434.0013221
AGATCAT200.002789453834.00131621
ATTATCA200.002789453834.00131621
GTTATTG301.4844038E-534.00131621
ACTTATT455.99357E-934.00071330
AAAGGGC200.002789697134.00071330
ATCAGTT200.002790102733.9997061
TATGACA200.002790102733.9997061
GATGGGT200.002790102733.9997061
TCATGTA2200.033.9997061
CGTAAAC200.002790102733.9997061
GGCTGAT200.002790102733.9997061
TAATGCA408.076677E-833.9997061
GTCATAC252.0293125E-433.9997021
AAATCGT1650.033.9997021
CAACGGG850.033.9997021
CAACGAA252.0293125E-433.9997021
CTATACG1400.033.9997021
Produced by FastQC (version 0.11.5)