FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11566979
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG187316716.194090090420325No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT10051468.689788405425478No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC8961777.747718743156705No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC6785045.865870423037856No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG3971323.43332515776159No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC3578033.09331416612756No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA2681692.3184013734268905No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT2357622.0382331462692203No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC2314122.0006260926037815No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1880721.6259388039003095No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG1041440.9003560912490635No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT1010510.8736161792979826No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC954100.8248480437286175No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA857800.7415938076830606No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC769450.665212584893601No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG726350.6279513432158907No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG649420.5614430526760704No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC648180.560371035514113No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG611900.5290058882271681No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG452100.3908539991297641No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG444140.38397234057397356No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT432390.3738141134344586No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACTAGATCGGA395320.3417659874717504No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT388040.33547220929509775No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC386530.3341667690414239No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG370670.3204553237280019No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA356620.30830867765904996No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG341270.2950381426299814No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA327470.28310762905335957No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG323500.27967544507515746No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT310380.2683328118776735No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT287230.24831894308790567No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA271860.2350311174594507Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG268790.232377010453637No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG253600.21924480021965975No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT243430.21045253043167106No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT241170.2084986927010069No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC235700.20376971376882416No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC231460.20010410669890558No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC226580.19588520044862187No Hit
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGT221780.19173545659588387No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA212530.18373855437966993No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC208980.1806694729885824No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT207680.17954558402846585No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT205960.17805859248123473No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG198080.1712460963229898No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT184210.1592550656485155Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG181490.15690354413196395No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG174380.1507567360500957No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG162660.14062444480966033No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC157860.1364747009569223No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAG155450.13439118373086006No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAG154960.13396756404589305No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC149310.12908296971923264No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC148260.1281752132514462No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT145030.12538278145054124No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC142840.1234894608177295No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA140390.12137136239289446TruSeq Adapter, Index 12 (100% over 39bp)
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA140260.1212589734968828No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGC133540.11544933210304956No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT131000.11325342598097568No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCA129700.11212953702085912No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG129670.11210360112177951No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT125790.10874922484081626No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT124510.1076426264800861No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC123260.1065619640184356TruSeq Adapter, Index 12 (100% over 40bp)
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT123020.10635447682579868No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACAGATCGGAA120100.10383004931538303No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCA301.4837064E-534.003811
GTCATAC252.02792E-434.003811
CAACGAA200.002788494634.003811
ATCAGTT351.0915282E-634.003811
CTATACG2800.034.003811
GTCTACG1200.034.003811
CGACATG301.4837064E-534.003811
TTTCCGG8050.034.003811
TAACGGA1600.034.003811
TGCGGGT200.002788494634.003811
TATTACA1400.034.003811
TAATCCT301.4837064E-534.003811
AAATCGT1150.034.0038071
TATGGAT750.034.0038071
TGTAAAC112550.034.0038071
CATGTAC3400.034.0032232
TAAACAC351.0916756E-634.003223
GGTTTAC9550.034.003223
GAGGTAC301.4838819E-534.003222
CATACAC252.028126E-434.003223
Produced by FastQC (version 0.11.5)