FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11143917
Sequences flagged as poor quality0
Sequence length40
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA10579939.49390595784229No Hit
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG9294968.34083742727086No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT9033268.10600078948901No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT6009075.392242243010245No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC4396583.9452734617459915No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC4142873.717606654823434No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC3575483.2084589287590712No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC3074262.7586888882966374No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA2022611.8149901870231089No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1789711.6059972449543551No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT1622211.4556910285674238No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1393821.2507451374592973No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA1300381.1668967024790295No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC1197061.074182444108297No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG976090.875894894048475No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG902890.8102088341110222No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG821610.7372721817651728No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA803250.720796825748074No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT754130.6767189669485154No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG715110.6417043486594525No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG608130.5457057872918473No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC583600.5236937784084357No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT581050.521405534517172No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG538320.4830617457039567TruSeq Adapter, Index 7 (97% over 36bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT530710.4762329080519893No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT522160.4685605608871638No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC470750.42242776933819587No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT467330.41935883047226574No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG398960.35800697367003004No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT397630.3568134974443905No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA362790.32554980443590886No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG359910.3229654348645992No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC333090.29889849323177836No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT330200.2963051501550128No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA320630.2877175054336819No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC316870.28434346738224986No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG305980.27457131994073536No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC291280.2613802669205092No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGA283260.25418351554484836No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC279160.2505043783079145No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA267720.24023868806632354No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG266580.2392157084443468Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC260900.23411875734537504No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG245610.22039826750324862No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG234690.21059919954536632No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT230190.20656112209019506No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA230170.20654317507928316No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG229960.2063547314647085No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT207960.1866130194616489No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG202650.1818480880645468No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT200890.18026875110430202No Hit
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT194910.17490259484165216No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG192580.17281176807041904No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC191140.17151958328476422No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAA182950.1641702823163525No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG182450.16372160704355568No Hit
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACG176210.1581221396390515No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC166630.14952552141226466No Hit
CCCCCCACTGCTAAATTTGACTGGTAGATCGGAAGAGCAC160420.14395297452412828No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA159720.14332482914221273No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACG151840.13625370684293503No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG149610.13425261512626127No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG144640.12979278291466098No Hit
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGT142640.12799808182347375No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT142500.12787245274709064No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT142420.12780066470344315No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG139060.1247855668702486No Hit
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGT137770.12362798466643281No Hit
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACG137720.12358311713915314No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG135300.12141152881881657No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAG135110.1212410322151538No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC131600.1180913318001202No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACAC126550.11355971154487243No Hit
CTAGACTGAAGCTCCTTGAGGAAGATCGGAAGAGCACACG123860.11114583857722558No Hit
CCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACA121110.10867812457684314No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA117750.10566302674364858No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC112030.10053018162285308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGCA2700.034.0076371
CGAATGT1350.034.0076371
AATCCGT252.026576E-434.0076331
AAATCGT2350.034.0076331
CGACGGA252.026576E-434.0076331
CATGACG200.002786953734.0076331
CATTGCG900.034.0076331
CTATACG3000.034.0076331
GTCTACG4400.034.0076331
ATCACAT700.034.0076331
TATTGCG2350.034.0076331
TCAATGC252.026576E-434.0076331
TCATGTA1450.034.0076331
TATTACA950.034.0076331
TACCTGG504.456524E-1034.0076331
ATTGGTT301.4825595E-534.0076331
CGCAACC200.002786953734.0076331
AATGTGT850.034.0076331
TATATGA301.4839721E-534.0029032
CCCACAT200.00278885534.0029032
Produced by FastQC (version 0.11.5)