FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7557386
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA335672344.416455636909376Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA4446515.883661361216696Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC1041601.3782543329135233Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC880221.1647148895133848Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA800891.059744731842465Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC709820.9392401023316793Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA578210.7650925862460909Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG560440.7415791650710973Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA489900.6482400131474031Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA489660.647922443024612Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC486570.6438337276936761Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC485880.6429207135906516Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC464900.6151597920233265Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC447720.5924270640668613Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA342630.45337104654969324Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA340690.4508040213904649Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA324150.4289181470947759Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG318460.42138908876693604Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG299230.39594378267829644No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC276950.3664626896125195Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC272740.36089198037522496Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG272540.36062733860623236TruSeq Adapter, Index 15 (97% over 49bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA230940.3055818506557691Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC205060.27133720574812503Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAA195830.2591239881091161TruSeq Adapter, Index 15 (97% over 39bp)
AAACCGTTACCATTACTGAGTAGATCGGAGAGCACACGTCTGAACTCCAG192750.2550485048666298Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC175380.23206436722962145Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC167810.22204767627325112Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC163250.21601384394021955Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC161510.2137114605499838Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA160360.21218977037827633Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA157330.20818044757803822Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC153600.203244878586326Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA144340.19099196468196808Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTAAACTCCA142410.1884381716111894Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCCGTC133720.17693948674846038TruSeq Adapter, Index 15 (98% over 50bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC128670.17025728208139693Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC123500.1634162923529379Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACCGAGTAGATCGGAAGAGCACACGTCTGAACTCCA120660.1596583792332428Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATCCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA118420.15669439142052558Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG116660.15436554385339057Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA115200.15243365893974453Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA113270.14987986586896582Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG106670.1411466874922096Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG106370.14074972483872067Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC102820.13605233343910184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGCTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA101170.13386903884491277Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCGTTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA98580.13044192793645845Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA97890.12952891383343396Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA96730.1279939915732768Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCACTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA95960.12697512076265524Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC87920.11633652164915224Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTGCCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA86050.1138621211090713Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCG85410.11301526744829495Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT82700.10942937147844507Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCC82570.10925735432859986Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AAACCGTTACCATTACTGAGTAGATCGGAAAGCACACGTCTGAACTCCAG81520.10786798504138866Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC81050.10724607688425601Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGACACACGTCTGAACTCCAG80060.10593610012774259No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC79270.10489076514022178Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGGTGCCCCCTCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA79150.1047319800788262Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA78060.10328968243781646Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATTGCACGGTATCCATCTGTAAGATCGGAAGAGCACACGTCTGAACTCC77120.10204586612355118Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTCACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA76420.10111961993207703Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTAGATTGGAAGAGCACACGTCTGAACTCCA75850.10036539089044809Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGGT302.521465E-644.0206031
TATGGAT302.521465E-644.0206031
GACCAAC302.521465E-644.0206031
GTTAAAC302.521465E-644.0206031
AATGACC302.521465E-644.0206031
AAACCGA2300.044.0206031
AATTACA3300.044.0206031
TTGATTC207.8411784E-444.02061
TTAGGTA254.432939E-544.02061
AATCCGT19450.044.02061
AAATCGT70350.044.02061
TTAATGC207.8411784E-444.02061
CGAAACT2000.044.02061
CATGCCT1600.044.02061
TAAACTA1500.044.02061
GAATCGT3150.044.02061
AATGGCG3850.044.02061
TTTCCGG1350.044.02061
TAGTTGC207.8411784E-444.02061
ATCAAGT207.8411784E-444.02061
Produced by FastQC (version 0.11.5)