FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497101
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC2026785.795600413027819Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA1709274.887676964434256Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC1177913.3682470137408096TruSeq Adapter, Index 8 (100% over 50bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA971152.7770144471091913Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC872782.495724315654595Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG868142.4824561829927134Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC588691.6833657363627759Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC515441.4739065300087129Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC406141.161361939503606Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA345620.988304312629232Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC331170.9469843736283281Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG329540.9423233701285721TruSeq Adapter, Index 8 (100% over 49bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC325160.9297987104175716Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG281330.8044663279670791Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA279650.7996623488998459Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC256960.7347800363787034Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA254070.7265160485785226Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC239940.6861111532094727Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC226400.6473933695366534Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG178910.5115951755468315No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA158340.4527750270867213Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC140420.4015325837029013Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC130290.3725657337320255Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA122100.349146335779264TruSeq Adapter, Index 8 (100% over 40bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC116160.3321608383629755Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC108890.3113721908517941Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC107240.3066539971250473Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGTCTGAACTCCA103340.29550190286182754Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC99120.2834347649667539Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC96020.27457027978316895Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG91470.26155950314274595Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTATGTCTTGTTGATCTCAATAGATCGGAAGAGCACACGTCTGAACTCCA90000.25735602145891695Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG85630.24485995686141176Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAATTTGGTGTTTCTTCGATCAGATCGGAAGAGCACACGTCTGAACTCCA83970.24011316802116955Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG83790.23959845597825172Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC81420.23282141407983356Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC78130.22341362173983534Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGTCTGAACTCCA77820.2225271732214769Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC70790.20242480843418592Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC70500.2015955501428183Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGTTCTCAGGTCACCCCTTAGATCGGAAGAGCACACGTCTGAACTCCA70090.20042315048950546Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC69460.19862165833929302Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGTCTGAACTCCA66440.1899859340636716Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC62910.17989185899978297Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC62350.1782905326440386Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCATGGTCAGATCCGTCATCCAGATCGGAAGAGCACACGTCTGAACTCCA59730.17079861290823456Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC58910.16845381360160888Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTCAGATCGGAAGAGCACACGTCTGAA55120.15761626558683892Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGTCTGAACTCCA54900.15698717308993937Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC54770.1566154366144987Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCAATATTACTGTGCTGCTAGATCGGAAGAGCACACGTCTGAACTCCAG54450.15570039298264476Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAAACTAATCACGGAAATGCAAGATCGGAAGAGCACACGTCTGAACTCCA53200.15212600379571536Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCAATATTACTGTGCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCA52040.14880897063024487Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA51860.14829425858732703Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATTGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA50130.14334730395261674Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGTCCGGTTTTGGATACGTGAGATCGGAAGAGCACACGTCTGAACTCCA49670.1420319287318267Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC48760.13942977340374213Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG48620.13902944181480603Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG48260.13800001772897036Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCAAATGTAGACAAAGCAAGATCGGAAGAGCACACGTCTGAACTCCAG48110.13757109102653886Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCCCACTGCTAAATTTGACTGGAGATCGGAAGAGCACACGTCTGAACT46400.1326813266188194Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC46200.1321094243489107Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC45740.1307940491281207Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA45710.1307082637876344Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGTCTGAACTCCA45070.12887817652392652Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA44910.12842065470799957Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCC43690.12493205086155648Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG43070.12315915382483948Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA41120.11758310669322963Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGAGAAAGGCAGTTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA40920.11701120442332091Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCAACCTTTCATCGTTCCCAGATCGGAAGAGCACACGTCTGAACTCCA40430.1156100438620446Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACGTCTGAACTCC40420.11558144874854917Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCA40380.11546706829456742Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT39970.11429466864125457No Hit
GCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC39700.11352260057687782Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACGTCTGAACTCC39540.11306507876095086Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC39260.11226441558307867TruSeq Adapter, Index 8 (98% over 50bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA39100.11180689376715171Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC38880.11117780127025212Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACGTCTGAACTCC38810.11097763547578407Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTTCGTGAATATCTGGCATAGATCGGAAGAGCACACGTCTGAACTCCA38450.10994821138994842Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTGCTATGTTGCTGCTCATAGATCGGAAGAGCACACGTCTGAACTCCA38240.10934771400654428Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACGTCTGAACTCC37720.10786076810478165Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAA37200.10637382220301901Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCA36930.10560175413864227Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAG36210.10354290596697094Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTCTGATACCCAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC36100.10322835971852114Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC35530.10159843824928134TruSeq Adapter, Index 8 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph