FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7315613
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA112218715.339616789461116Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC5960368.147451211538938Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA2760853.773914776519753Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC2345013.2054866762361542Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC1888882.5819845855706145Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG1761012.4071940382849664Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC1636192.2365726563173856Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA1394801.9066071428327331Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1368861.8711487335374355Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC1002471.3703157889844637Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC517030.7067487030820248Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC508660.69530741989769Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG446650.6105435046933182Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC399160.5456275502818424Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG394430.5391619266902172No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC393630.53806837513138Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC365160.49915160903125955Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC312790.4275649901108766Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA264400.36141879019570883Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC259530.3547617950812871Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC258210.3529574350092057Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA249340.34083268210059775Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG247120.33779807652482435TruSeq Adapter, Index 6 (100% over 49bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC246380.3367865413328999Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG242540.33153749385048115Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC221020.3021209569177593Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC209600.2865105084153577Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC193210.26410637085367966Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC186620.25509823988775787Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG183580.2509427439641763Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA180150.24625414165566167Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG180050.246117447710807Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA177540.24268642969495516Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA174480.2385035949824027Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA171640.2346214869485305TruSeq Adapter, Index 6 (100% over 40bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC168590.2304523216304635Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA165770.2265975523855622Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT164770.22523061293701568Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA164360.2246701677631116Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC163720.22379532651604178Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC159890.21855994842810847Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC159530.21806785022663172Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA157690.21555268164130606Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA155490.21254541485450368Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC144680.19776879941571537Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGTCTGAACTCCA141180.19298451134580247Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC141140.1929298337678606Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA138310.18906139512847384Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGAGATCGGAAGAGCACACGTCTGAACTCCAG128930.1762395031011072Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTACACTCTCAGCAGATCGGAAGAGCACACGTCTGAACTC125420.17144154563670877Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTCTCACTACTGCACTTGACTAGTCAGATCGGAAGAGCACACGTCTGAA124840.17064872075655177Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC123740.16914508736315056Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG122940.1680515358043133Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC121830.16653423301642667Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA119090.1627888189274091Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC117750.1609571200663567Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC117570.16071107096561835Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACACGTCTGAACTC112910.15434113313539138Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACGTCTGAACTCC111250.1520720136508041Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG109510.14969353901033308Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC103360.1412868614017718Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA101940.13934580738483568Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGAGAAAGGCAGTTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA101170.13829326400945485Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA100390.13722705123958853Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACGTCTGAACTCC100370.1371997124506176Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAG98760.13499893993845763Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA98530.13468454386529194Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC97360.13308522471049247Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGAGATCGGAAGAGCACACGTCTGAACT96810.13233340801379187Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC94580.12928513304353306Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC90920.12428213466185267Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC88270.1206597451232043TruSeq Adapter, Index 6 (100% over 50bp)
TAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA84460.11545170582424194Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCA83310.11387972545841339Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC83020.11348331301833489Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATGGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC81990.11207536538633195Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC79730.10898608223261673Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACGTCTGAACTCC79320.10842563705871265Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA76700.10484425570352067Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA75720.10350465504394504Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA75270.10288953229209911Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGG700.044.021161
TTTCGCA454.783942E-1044.021161
GAATCGT1850.044.021161
TGTGCAT207.84067E-444.021161
AATTGCG408.287316E-944.021161
AAACAGT650.044.021161
TAAGCGT1650.044.021161
TAATGTC207.84067E-444.021161
TTAGGTA600.044.0211561
GGTAAAC12100.044.0211561
TATTGCG2400.044.0211561
AAAAAAC254.432595E-544.0211561
AAGCCGT8250.044.0211561
TAACCGT33800.044.0211561
GTATGTA254.432595E-544.0211561
TCATGTA2350.044.0211561
ACCAATC254.432595E-544.0211561
TCGAATT551.8189894E-1244.0211561
TATTACA2300.044.0211521
GGTGTAC408.314601E-944.0055122