FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4190123
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC52236712.466626874676471Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA3796709.06107052227345Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG1901714.538554118817037Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1715994.0953213067969605Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC1107022.6419749491840694Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC1019652.433460783848111Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC973822.3240845197145763Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG568631.3570723341534365Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA462961.1048840332372105Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA444811.0615678823748134Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC437041.043024273989093Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC415990.9927870852478555Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC389070.9285407612139309Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC385940.921070813434355Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC363780.8681845377808719Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG324780.7751085111343986Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC312700.74627880852185Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC269880.6440861043935941Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA260220.6210318885626985Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC209000.4987920402336638Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC177910.424593741042924Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG176620.42151507246923303No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA157260.3753111782160094Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACACGTCTGAACTC153110.36540693435491034Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC148760.35502537753664987Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC140470.3352407554623098Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC135610.32364205060328777Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC131800.3145492387693631Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC128220.306005336836174Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGAGATCGGAAGAGCACACGTCTGAACTCCA126550.30201977364387633Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGTCTGAACTCCA126410.30168565457386337Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAG121700.29044493443271235Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA116590.27824958837723857TruSeq Adapter, Index 1 (100% over 40bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC115370.27533797933855403Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA106160.2533577176612715Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGTAGATCGGAAGAGCACACGTCTGAACTC104890.25032678038329664Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG103380.24672306755672804TruSeq Adapter, Index 1 (100% over 49bp)
GCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG100300.23937244801644245Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA98520.23512436269770598Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC97060.23163997811042777Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGTCTGAACTCCA91690.21882412521064418Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA89900.21455217424404963Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA88560.21135417743106824Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC87040.2077265989566416Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA85550.20417061742578918Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC83430.1991111000798783Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA77970.1860804563493721Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG77300.1844814579428814Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC77140.18409960757715227Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC74880.17870597116122844Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGTCTGAACTCCA74210.17710697275473775Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG73360.1750783926868018Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG72670.17343166298459495Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACACGTCTGAACT68700.16395700078494116Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC66060.15765646975041067Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACGTCTGAACTCC63120.15063996928013806Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG63040.15044904409727353Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAAGAATTCTCCTTTTGGGAGATCGGAAGAGCACACGTCTGAACTCCAG62730.14970920901367335Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC58480.13956630867399358Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC56700.13531822335525712Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG53870.1285642450114233Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC53420.12749029085781016Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC52540.12539011384629997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA52300.1248173382977063Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC52120.12438775663626105Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTATACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA51990.12407750321410613Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGGAGATCGGAAGAGCACACGTCTGAACTCCAG51370.12259783304690579Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC51150.12207278879402825Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA50650.12087950640112474Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC49820.11889865762890492Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCCGGCATCTCCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC49510.11815882254530477Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA47850.11419712500086512Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA46850.11181056021505813Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGTCTGAACTCCA46440.11083206865287726Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTGAGATCGGAAGAGCACACGTCTGAACTC46350.11061727782215464Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCTACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC44950.10727608712202481Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC43590.1040303590133273Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACACGTCTGAACTC43420.1036246429997401Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG42430.10126194386179117Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGGCA1450.044.0180441
TATTTCC254.4341214E-544.018041
AAACTGT1100.044.018041
AATCCGT900.044.018041
GAATCGT254.4341214E-544.018041
CTCTTAC351.4426587E-744.018041
GTCTACG700.044.018041
TATGACA551.8189894E-1244.018041
TCAATGC254.4341214E-544.018041
AAGCCGT3250.044.018041
AATTGCG254.4341214E-544.018041
TCATGTA1700.044.018041
GGAACGC351.4426587E-744.018041
TCATGCT750.044.018041
GTCAAGT351.4426587E-744.018041
CGAATGT551.8189894E-1244.018041
AAACAGT254.4341214E-544.018041
GATGCCT454.783942E-1044.018041
TTTTGCA3700.044.018041
CGCAACC4600.044.018041