FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2437713
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC26861611.019180682877764Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1100214.513287659375816Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG990964.065121693981203Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA875583.591809208056896Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC621902.5511616831021535Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC431651.7707170614424257Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC347121.4239576192931653Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC344851.4146456125064764Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA315401.2938356566174936TruSeq Adapter, Index 1 (100% over 40bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC235240.9650028530840177Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC232240.9526962361853097Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG218360.8957576220006211Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG195940.8037861717109438Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC180390.7399968741193077Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA175960.721824103165549Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA173660.712389030209873Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC159390.6538505558283523Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC143240.5875999348569746Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC142700.5853847438152071Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG122340.5018638371293094TruSeq Adapter, Index 1 (100% over 49bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA113520.465682383447108Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC103970.42650631965288777Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC95570.39204779233650555Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA94730.38860193960486733Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG93990.3855663074365194No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGTCTGAACTCCA90780.3723982273549019Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC90590.3716188082846504Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACACGTCTGAACTC88600.36345541907517415Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC86470.35471772107709154Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC85940.3525435520916531Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC79150.3246895758442442Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA72020.29544084968164835Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC71000.29125659993608766Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA68150.2795653138823151Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC67690.2776782992911799Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA67190.27562719647472855TruSeq Adapter, Index 1 (100% over 40bp)
GCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA63210.259300418055776Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC62170.25503412419755733Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG62110.2547879918595832Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC61300.251465205296932Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC60150.24674766881909396Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG57590.23624602239886322Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACGTCTGAACTCC54660.22422655989445847Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG52600.215776016290679Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGTCTGAACTCCA52050.21351980319258257Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGTCTGAACTCCA52000.21331469291093744Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGTCTGAACTCCA51100.20962270784132503Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAG49550.20326428911032596Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC48320.1982185761818557Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC47810.19612645130907533Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG47430.19456761316857235Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAAGAATTCTCCTTTTGGGAGATCGGAAGAGCACACGTCTGAACTCCAG45890.18825021649390228Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC39790.16322676213319615TruSeq Adapter, Index 1 (100% over 50bp)
CAACGGAATCCCAAAAGCAGCGAGATCGGAAGAGCACACGTCTGAACTCC38920.15965784323257087Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA38480.15785287275409368Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG37400.15342249067055885Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGTAGATCGGAAGAGCACACGTCTGAACTC37210.15264307160030732Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC36880.15128934374144945Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC36170.1483767777420886Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCC36140.14825371157310152Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAACT35850.14706407193955973Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCTCACTACTGCACTTGACTAGTCAGATCGGAAGAGCACACGTCTGAA35410.1452591014610826Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC34320.1407876973212187Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC33620.13791615337818686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC32420.13299350661870368Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACGTCTGAACTCC30030.12318923515606636Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGAGATCGGAAGAGCACACGTCTGAACTCCA28920.11863578690354444Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA28770.11802045605860903TruSeq Adapter, Index 1 (100% over 40bp)
CCCCCCACTGCTAAATTTGACTGGTAGATCGGAAGAGCACACGTCTGAAC28580.11724103698835754Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG28360.11633855174911895Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA28330.11621548558013188Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACGTCTGAACTCC28240.11584628707317063Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA27990.11482073566494497Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA27790.11400029453836444Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC27700.1136310960314032Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG27040.11092364031368745Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAAGATCCGGACTACAACAAAAGATCGGAAGAGCACACGTCTGAACTCCA26610.10915969189153932Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCCGGCATCTCCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC26250.10768289786369438Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA25940.10641121411749455Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC25760.10567281710357208Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCA25660.10526259654028182Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA25070.10284229521686926Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC24940.1023090084845919Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGGGCGGAAACACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC24780.10165265558332748Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGTCTGAACTCCA24460.10033994978079863Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGT2650.044.0143051
TTAGGTA207.8456546E-444.01431
CATGGAT207.8456546E-444.01431
TTAGGGG254.4357963E-544.01431
ACCGGTA551.8189894E-1244.01431
CGAAACT1600.044.01431
TTTAAAC700.044.01431
ATCAGTT302.5232403E-644.01431
CTATACG800.044.01431
TATGGCA1500.044.01431
CTATACA207.8456546E-444.01431
TTATGGG254.4357963E-544.01431
CCGTGTG254.4357963E-544.01431
TAAACTA750.044.01431
GTCTACG600.044.01431
AATGGCG650.044.01431
TCCTATA1050.044.01431
ACCCTAT207.8456546E-444.01431
TATTGCC254.4357963E-544.01431
GGGAATT207.8456546E-444.01431