FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8488802
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA254284929.955334097791418Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC4161884.902788402886532Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA3306033.8945778214640887Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA2327862.7422715242975393Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1927872.271074292933208Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1910502.2506120416049287Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC1284191.5128047514831893Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC1250341.4729286888774176Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG883101.0403116953369862Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG793500.9347608767409112Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC712680.8395530959492282Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC600640.7075674518029752Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC594590.7004404155026822Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA569070.6703772805632644Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA551700.6499150292349851Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA438950.5170929890931606Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC434690.5120746131197311Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC395940.46642624012198663Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC340380.4009753084121882Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC333370.3927173704840801Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA329450.3880995221705018Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT305700.360121487107368Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG304300.35847225556680434Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG254080.29931196416172745Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA253490.2986169308696327Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA246610.29051213587029123Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC221640.2610969133218091Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC209360.24663079666600776Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA196440.23141074559166303Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACGTCTGAACTCC191650.22576801767787727Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC190850.22482559965469806Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG177290.20885161416180986TruSeq Adapter, Index 7 (97% over 36bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA171200.2016774569603579Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG162980.19199411177219117Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC152780.17997828197665586Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC152300.17941283116274828Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC140620.16565352802433136Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC139640.16449906594593677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA135140.15919796456555355Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCCGTC134020.1578785793331026RNA PCR Primer, Index 23 (100% over 34bp)
TAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA133100.1567947986064465Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA130120.1532842914701038Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA122690.14453158407982658Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC122390.14417817732113436Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC121490.1431179570450577Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA119470.14073835153653014Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA118190.13923048269944335Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACGTCTGAACTCC118120.13914802112241514Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC117640.1385825703085076Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC116690.13746344890598225Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA113660.13389404064319088Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA108580.1279096861960027Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA108450.12775654326723607Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCAGATCGGAAGAGCACACGTCTGAACTC107680.12684946591992602Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG106500.12545939933573666No Hit
AAACCGTTACCATTACTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG102720.12100647417621474Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA101360.11940436353681003Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC98440.11596453775220578Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC91980.10835451221503341Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG90790.10695266540555429Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC90440.10654035752041335Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT89270.10516207116151371Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC88290.10400760908311915Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACGTCTGAACTCC88140.10383090570377305Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACCGAGTAGATCGGAAGAGCACACGTCTGAACTCCA87420.10298272948291172Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA85930.10122747591474039Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC302.5221962E-644.018841
TATTTCA502.7284841E-1144.018841
AAATCGT73600.044.018841
CTATACG2300.044.018841
GGATCGT254.434032E-544.018841
AATGGCG4100.044.018841
TTTCCGG2400.044.018841
TATTGCG2100.044.018841
AATTGCG502.7284841E-1144.018841
AAACCTT1550.044.018841
TCATGTA1650.044.018841
CGAATCA1500.044.018841
AATTCGT254.434032E-544.018841
CATCGGG254.434032E-544.018841
CGTAAAC1550.044.018841
CAATGGA7500.044.018841
AAGGCGT302.5221962E-644.018841
TGGCTTG1050.044.018841
CTCTACC254.434032E-544.018841
CAACGGG800.044.0188371