FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6798416
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA143069821.04457861949019Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC4347856.395386807750511Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA2449863.6035747150512707Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1907122.8052416915940417Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA1891842.7827658678139144Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC1862572.739711721083264Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1606592.3631828355311004Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG1392392.048109441964128Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC1074401.5803681328121137Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC1026831.5103959510568346Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC575760.8469031609716146Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC416780.6130545703587423Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC416360.612436779390964Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG405240.5960800280535937Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC389300.5726333898955286Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG386500.5685147834436728No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATG353330.5197240062979377TruSeq Adapter, Index 19 (97% over 49bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG298610.43923466878166917Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC279110.4105515167062445Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC264180.38859051873259887Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA228590.3362400888677598Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA217720.3202510702493051Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC214290.3152057773457817Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT203810.2997904217688356Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC203770.29973158453380905Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA196970.289729254579302Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC193470.2845809965144822Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAA179680.26429685973909217TruSeq Adapter, Index 19 (97% over 39bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC178900.2631495336560752Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACGTCTGAACTCC161330.23730527817067976Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG158780.23355440443773962Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC154070.2266263200133678Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA149670.2201542241604515Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA149570.2200071310728852Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA146390.21532957088827748Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC145720.21434404720158343Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA141810.20859270747774186Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC136090.20017898286895067Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGCCGTC134340.19760485383654075TruSeq Adapter, Index 19 (98% over 50bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA129790.19091211835227498Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA129320.19022078084071348Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA126390.18591095337502148Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA126050.18541083687729612Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC121320.1784533338354111Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG114640.16862751558598355Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC111970.16470013014796386Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC109350.16084629125372735Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC104970.15440361401832428Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA103490.15222663632234332Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC102400.15062332166787087Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC101600.14944657696734062Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC101230.1489023325433454Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA100140.14729901788887292Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA91790.13501674507708855Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAG90770.13351639558391248Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACACGTCTGAACTC88130.1296331380721627Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC84810.1247496475649622Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC84750.12466139171242241Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACGTCTGAACTCC84170.123808251804538Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCC83140.12229319300260531Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACGTCTGAACTCC80280.1180863306982097Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGTCTGAACTCCA78290.11515917825564073Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCAGATCGGAAGAGCACACGTCTGAACTC76240.11214376996053198Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC76190.11207022341674884Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG76030.1118348744766428Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT74520.10961376885439196Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC74470.10954022231060882Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGTCTGAACTCCA73420.10799574489116288Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT70610.10386242913055041No Hit
CAACGGAATCCCAAAAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCA69350.10200905622721529Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGAGAAAGGCAGTTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA68160.10025864848517654Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA68010.10003800885382713Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAAAC254.4314715E-544.022991
CATTGCG351.4415673E-744.022991
GAATCGT1150.044.022991
GTCTACG1000.044.022991
TGCGGGT254.4314715E-544.022991
TCATGTA5150.044.022991
AAACAGT1150.044.022991
TTGATTC207.8390265E-444.0229871
CAACGGG1100.044.0229871
TATGGCA10400.044.0229871
TATGGAT454.783942E-1044.0229871
AATGGCG5100.044.0229871
CGACATG207.8390265E-444.0229871
GTGGCAT207.8390265E-444.0229871
ATCACAT1550.044.0229871
AAAGTTC207.8390265E-444.0229871
TATTGCG2400.044.0229871
AATGATA207.8390265E-444.0229871
GTTAAAC408.283678E-944.0229871
TCATGCT5250.044.0229871
Produced by FastQC (version 0.11.5)