FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5843491
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG71888612.302337763504728No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT4014846.870618950213152No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC3451515.90658905780808No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC2464594.217667144520288No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT2386724.084407762414624No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG2108193.607757759873336No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC1450352.481992356966067No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG998561.7088415127190237No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC931991.5949198860749507No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG628561.0756583692864419No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT564550.9661176854726052No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG557220.953573814009468No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC380770.6516139068238489No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA378100.6470447203563761No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG370100.6333542740118878No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA369680.6326355255788021No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC367490.6288877658919985No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT335680.574451128614727No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT297720.5094899607101303No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC292970.5013612581930904No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG268480.45945137932102575No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT258880.44302284370763984Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG213870.3659969699619628No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT207710.35545532627670684No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG202500.3465394230948589No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT195310.3342351344427501No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG188530.3226324811657963No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC167930.287379581828739No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT165250.28279328230333545No Hit
TCCGGGTGCCCCCTCCAAGATCGGAAGAGCACACGTCTGA160680.27497261482904656Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT153120.2620351430335051No Hit
AAACCGTTACCCTTACTGAGTAGATCGGAAGAGCACACGT149550.2559257813522773No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG148810.25465941506541206No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG143040.24478518063944993No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC142490.24384396245326637No Hit
TCCCCGGCATCTCCACCAAGATCGGAAGAGCACACGTCTG137680.2356125815886428Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG136020.2327718139721615No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA135720.23225842223424323No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT127230.21772943605115505No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC121510.20794076691484595No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAA117130.2004452475412386TruSeq Adapter, Index 19 (100% over 39bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG116270.19897352455920614No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG110940.18985226468219082No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT110910.189800925508399No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT109860.1880040544256849No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG109460.1873195321084605No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC109010.18654944450158306No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC107970.18476968647679956No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC106440.1821513886134162No Hit
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT103310.17679500148113517No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG101330.17340661601087431No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT101180.17314992014191516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT101140.17308146791019272No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTC100510.17200334526056427TruSeq Adapter, Index 19 (97% over 40bp)
GGCTGGTCCGATGGTCGTGGGTTATCAGAACTAGATCGGA96580.16527791349383442No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT96150.16454205200281818No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG94990.16255693728286738No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC93300.15966483049259422No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT92620.15850114255331274No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA89500.15316186847896232Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG88730.15184416301830533No Hit
CAACGGAATCCCAAACGCAGCTAGATCGGAAGAGCACACG86980.14884937788044852No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGT86740.14843866449011386No Hit
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACG86310.14770280299909763No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG82450.14109716263788205Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT80500.13776011634141302Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGAGAGAAAGGCCGTTCCTGAAGATCGGAAGAGCACACG80010.13692157650281314No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT78780.13481667037734807No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC78110.13367009549599718No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT78020.13351607797462167No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT76420.13077798870572402No Hit
CAAAGAATTCTCCTTTTGGGCTAGATCGGAAGAGCACACG73690.12610612389066742No Hit
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGT73490.1257638627320552No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACG72740.12448038338725943No Hit
ACCGGGCGGAAACACCAAGATCGGAAGAGCACACGTCTGA72130.1234364868534922Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA71740.1227690775941984No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC69200.11842236087982337No Hit
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACA68500.11722444682468065No Hit
TCCCCGGCACCTCCACCAAGATCGGAAGAGCACACGTCTG66950.11457192284543606Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC64160.10979737968279576No Hit
TCCTGTACTGAGCTGCCCCGGGAGATCGGAAGAGCACACG62140.1063405419808125No Hit
ATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACG62010.10611807222771455No Hit
TGTAAACATCCCCGACTGGAAGATCGGAAGAGCACACGTC61520.10527953238911465No Hit
TGGAGAGAAAGGCAGTTCCTGAGATCGGAAGAGCACACGT59990.10266123452573128No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA59290.10146332047058855No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG58700.10045365005268254No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTAA200.002788345334.00390232
CTTATTA850.034.0030331
TCAATTG301.4842484E-534.00157527
TATTTCA252.0286822E-434.0012861
TGAAGGG301.4843354E-534.0012861
ACTCTTT1500.034.0012861
CTATACG1350.034.0012861
GTCTACG1450.034.0012861
CGACATG252.0286822E-434.0012861
TCCTATA2300.034.0012861
ATCACAT1300.034.0012861
TATTGCG2200.034.0012861
TTATAAA301.4843354E-534.0012861
AATGACA25000.034.0012861
TCATGTT301.4843354E-534.0012861
TATTACA553.274181E-1134.0012861
AAACAGT252.0286822E-434.0012861
CGTAAAC553.274181E-1134.0012861
CAGAGTC252.0286822E-434.0012861
AAATCGT1750.034.001281
Produced by FastQC (version 0.11.5)