FastQCFastQC Report
Wed 8 May 2024
EGAF00005488406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6381935
Total Bases255.2 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG135020221.156624127321884No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT64373810.086878039340732No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC5186698.127143256708193No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC4781017.491473980853769No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC3592755.629562193911408No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1584612.482961672282779No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1501772.3531577805164106No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1122191.7583851919519708No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC908341.4232987330645015No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG792251.2413946553827326No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT607500.951905652439268No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC490840.769108428713235No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG461480.7231035728192156No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT387950.6078877331091589No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT341830.5356212496680083No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC329200.5158310136345795No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC296950.46529775060385287No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG254600.3989385664379221No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT240870.37742471523135224Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT235790.3694647469772099No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG231870.36332240926928905No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC222280.34829561880526827No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT219330.34367319629548093No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG218780.3428113887089104No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC216460.3391761276164674TruSeq Adapter, Index 4 (100% over 40bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA211930.3320779669488956No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT192100.3010058861458163No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC187500.2937980408763173No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC167840.2629923369636325No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG166830.2614097448501121No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG159180.24942278478235833No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT151900.23801558618193386No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC149270.23389457899524205No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT146860.23011829484317842No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT134390.21057876647129747No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG126110.1976046449861993No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA117330.1838470620587643TruSeq Adapter, Index 4 (100% over 39bp)
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT109120.1709826251755933No Hit
TCCTGTACTGAGCTGCCCCGGGAGATCGGAAGAGCACACG108240.16960373303708046No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT108230.1695880638082337No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT105030.1645739105772779No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA102490.16059392645020673No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC95030.14890468173054097No Hit
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG80200.1256672153508301No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA78610.12317580796419896Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT77540.12149920047759809No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG74530.11678276259473029No Hit
CCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG72030.11286545538304604No Hit
TATTGCACTTGTCCCGGCCTGGAGATCGGAAGAGCACACG67190.10528154862122538No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC66450.10412202568656685No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)