FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4486235
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG90916620.265679350279242No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC4026098.974318108614462No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT3866558.618696969730744No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC2334015.202603073624097No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC2060164.5921803026368435No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1762773.929285915695455No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1736133.869904273851013No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT1438823.207188210158407No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC1199992.6748264413255214No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA717251.5987793773620866No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT647141.4425013402106666No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC467981.0431464245631359No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC411540.9173393725473588No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG362420.8078488977951446No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG326790.7284281808688131No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC286090.6377062280509158No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT265720.5923006708297716No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG245200.5465607575171608No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG212360.4733590638921055No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT196440.4378727373844661No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG174840.3897254602132969No Hit
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGT159650.35586633335079415No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC150910.33638451842134887No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA141100.3145176300394429No Hit
CTAGACTGAAGCTCCTTGAGGAAGATCGGAAGAGCACACG136800.30493275541740456No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT121080.26989223703172033No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT118730.2646539915987459No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG117310.2614887539328635No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA98130.21873575503735315TruSeq Adapter, Index 12 (100% over 39bp)
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC92310.2057627386884548No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG86160.19205413893833026No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA85240.190003421577336No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG83980.1871948304090178No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT83480.18608031010412962No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT83150.18534472670290345No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC80850.18021793330041783No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA79850.17798889269064147No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC78070.17402120040523958No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT75500.16829256603811438No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG75320.1678913387283546No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG75040.16726720735761724No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC73460.16374532319417062No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC69190.1542273197904256No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACG67550.15057169319039237No Hit
CTAGACTGAAGCTCCTTGAGGTAGATCGGAAGAGCACACG66640.1485432662354959No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT65360.1456900942549822No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGTAGATC64670.14415205623423652No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG61060.1361052196329439No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG60360.13454489120610044No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT60020.1337870173987765No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC57950.12917290333653944No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC57700.12861564318409535No Hit
TCCTGTACTGAGCTGCCCCGACAGATCGGAAGAGCACACG57120.12732279963042506No Hit
TCCTGTACTGAGCTGCCCCGAGTTAGATCGGAAGAGCACA56460.12585163282797268No Hit
CTAGACTGAAGCTCCTTGAGGAAAGATCGGAAGAGCACAC56220.12531666308162637No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT55910.12462566049259569No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA52300.11657882389130307No Hit
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG50390.11232135632663023No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG48900.10900008581806346No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT46750.10420764850704434No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG46120.10280335292288521No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)