FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10005820
Sequences flagged as poor quality0
Sequence length40
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG8773418.76830684541597No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA7852377.847802578899081No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT7485857.481495769462174No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT5448965.445790549899958No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC4225844.223381991680842No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC4011854.009516461419453No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC3444853.4428462634746575No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC3005563.003811781543142No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1667461.6664901027601935No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA1557111.5562042891037415No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT1405811.40499229448461No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1391751.3909404726449206No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA1042011.0414039029284956No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG1013121.0125307071284513No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC1005981.0053948601913685No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG917690.9171562150828217No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG902290.9017651726695063No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG716090.7156734780357832No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG669900.6695103449792221No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT600080.5997309565832686No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC592690.5923452550615542No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA591460.5911159705051661No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACG589670.5893270116792028TruSeq Adapter, Index 22 (100% over 39bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT524770.5244647615088018No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT524440.524134953457088No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG483800.4835185921793516No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT479780.4795009304584732No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA422440.4221942829273363No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC399470.3992376436913716No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT380170.37994886975780096No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT336530.33633425346448365No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT322450.32226244325802383No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC314080.31389731176455304No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG312140.3119584401878107No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC311700.3115186961188588No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT300850.3006750071458411No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT298570.2983963333339996No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGA284970.28480424393003273No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG274490.27433033974227No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT270990.27083237555742556No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG261090.2609381340060085No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG259310.2591591693634305No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG251620.251473642340158No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC251610.2514636481567728No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG246440.2462966553465883No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG244510.24436777795323125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC241140.2409997381523953No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA225160.2250290331027342No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC224100.22396964966389563No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA213650.2135257280262887No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC200110.19999360372263345No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT194630.1945167912275056No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG189810.18969959483580556No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG186070.18596177024971466No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA183540.18343324185324142No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC181660.1815543353768107No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT179440.1793356266652808No Hit
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACG179120.17901581279695217No Hit
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT162610.16251541602787178No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC157770.1576782312694012No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACG156690.15659885946379207No Hit
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGT148910.1488233847900522No Hit
TATTGCACTTGTCCCGGCCTGTGAGATCGGAAGAGCACAC146770.14668462954560446No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG143010.14292681659274303No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG140410.14032832891257288No Hit
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGT130930.13085384306333714No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC130870.13079387796302552No Hit
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACG121470.12139934558087194No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAA120380.12030997959187753No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA120280.12021003775802483No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC109900.10983607540411482No Hit
CTAGACTGAAGCTCCTTGAGGAAGATCGGAAGAGCACACG109760.10969615683672103No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG108780.10871672686496459No Hit
TCGCTCTGATACCAAATTGCTGAGATCGGAAGAGCACACG106030.1059683264340154No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT103170.10310998998582825No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGC103010.10295008305166392No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT100450.10039157210503487No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGG252.0266505E-434.007381
CGAATGT455.982656E-934.007381
CGACGGA601.8189894E-1234.0073781
CTATACG4250.034.0073781
TATGGCA2900.034.0073781
CTATACA200.002787042434.0073781
ATATGTG200.002787042434.0073781
GTCTACG2200.034.0073781
TAACGGA200.002787042434.0073781
TCATGTA1300.034.0073781
AAACCGC2550.034.0073781
GTAAATG351.0906151E-634.0073781
TATCCGC200.002787042434.0073781
CTTGCAC200.002788545334.003642
CCTATAC2850.034.003642
TCACGTA200.002788545334.003642
CCCATAT3050.034.0036352
TCGTTCG504.456524E-1034.0021125
CTATACT2850.034.002113
CGTTCGG504.456524E-1034.0021126
Produced by FastQC (version 0.11.5)