FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8095209
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG151525818.7179602157276No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT89037510.998789530943549No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC6293017.773746175052429No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC4806635.937623105221866No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT2714863.353662641693377No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC2470453.0517433212657017No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG2131112.6325571087787853No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG2019852.4951177912762965No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1285121.5875068821570884No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT730000.9017679469424447No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC692780.8557901346339545No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG691090.8537024800718548No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC607230.7501103430436448No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA436750.5395166449686475No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG415970.5138471409447242No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG387740.4789746626677581No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT370290.4574187028401614No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT359740.4443863030590069No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG341410.4217432805008493No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG288620.35653186965277855No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT286120.3534436232591401Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT273280.3375823897814127No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC271600.3355070882048876No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA256450.3167923150594382No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG246520.3045258003839061No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT208580.25765857311404805No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG196960.2433044038764163No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG193650.23921556565123892No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC186370.2302225921529636No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC183930.22720846367277236No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT182290.2251825740385455No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT180680.2231937433610423No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC177950.21982137829918905No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG173700.21457135943000358No Hit
AAACCGTTACCCTTACTGAGTAGATCGGAAGAGCACACGT169650.20956840027230922No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC162190.20035307303369188No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT148500.18344183578212744No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA144840.17892064306184066Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT141480.1747700399087905No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT139260.1720276771112395No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG127940.15804409744084433No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC122590.15143525015845793No Hit
TCCTGTACTGAGCTGCCCCGGGAGATCGGAAGAGCACACG121430.15000230383180965No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG120950.14940936052423107No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA119380.14746994178902606No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT115810.1430599259389103No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT113720.14047815195382848Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTCGTGGGTTATCAGAACTAGATCGGA111200.1373651995890409No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT110090.13599401819026538No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC108920.13454871887804257No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGC108800.1344004830511479TruSeq Adapter, Index 22 (97% over 37bp)
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG105420.13022517392694866No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG99500.12291220646681265No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC98020.12108396460177866No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT94220.11638983008344812Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT92430.11417864566560296No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT88130.10886686186854472No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC86800.10722391478712903No Hit
TCCCGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG83250.10283860490816234No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG81630.1008374212450846No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC81430.1005903615335935No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)