FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3224481
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC2267427.031891333830158Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA2096576.50203862264966Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1518364.708850819713312Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1090143.380823146422634Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG1026853.184543497077514No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC706762.1918566119632894Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC579591.7974675614463225Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG532701.6520488103356787Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC518921.609313250721589Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC487511.511902225505438Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA380091.178763342069623Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC285330.8848865910513971Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG249390.7734267933351134TruSeq Adapter, Index 1 (100% over 49bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT214010.6637037092170802No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG201650.6253719590842681No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG194690.6037870900774419No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG189640.5881256549503625No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG189350.5872262854084115Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC157410.48817158482248774Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA151950.47123862723954646Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC137780.42729357065524654Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC135380.4198505123770306Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC131530.40791060638905924Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA128010.3969941209143425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA122330.37937888298923145Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACACGTCTGAACTC118100.36626049277387585Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA116520.3613604794073837Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACGTCTGAACTCC104660.32457936641586665Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC103150.31989644224915575No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC102810.3188420089930752Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC102730.318593907050468Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC97290.3017229749531785Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTC96800.3002033505547094No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACG96150.2981875222710259No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA95040.2947451078173511Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGAAGAGCACACG94360.2926362413051899No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGT88410.2741836593237795No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG87520.2714235252122745Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGTCTGAACTCCA84690.26264691899254483Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGTCTGAACTCCA83750.2597317211669103Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT83250.2581810840256153No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC82150.2547696823147663Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC80690.25024182186218497Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACGTCTGAACTCC78140.24233357244158052Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGAGATCGGAAGAGCACACGTCTGAACT71840.22279554446126368Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC66960.2076613259622246Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC59920.1858283550127912Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC59050.18313024638693792Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA57880.17950175547630765Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA54120.16784096417376937Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC53990.16743779851703267Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC53110.1647086771483535Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC52610.1631580400070585Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTAGATCGGAAGAGCACACGTCTGAA51870.1608630970379419Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC51680.16027385492424984No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC50240.15580801995732027Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC50040.1551877651008023Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA48260.1496674968777921Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA47920.14861306362171153Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC47770.14814787247932304Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT45820.14210038762827257Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC44910.1392782280311157Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCGAGATCGGAAGAGCACACGTCTGAACTCC44590.1382858202606869Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA44340.13751050169003942Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTCTCACTACTGCACTTGACTAGTCAGATCGGAAGAGCACACGTCTGAA43600.13521555872092283Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC42680.13236238638094006Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACGTCTGAACTCC40110.12439211147468383Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT39720.12318261450447374Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT39100.12125982444926796No Hit
ACCAATATTACTGTGCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCA38880.12057754410709817Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC38490.11936804713688808Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG38180.1184066521092852Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC37360.1158636071975614Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGTCTGAACTCCA37270.1155844925121283Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC37110.11508828862691392Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG37070.11496423765561031No Hit
CCCCCCACTGCTAAATTTGACTGGTAGATCGGAAGAGCACACGTCTGAAC36990.11471613571300311Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCC36970.11465411022735131Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC36720.11387879165670382Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA36710.11384777891387793No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACGTCTGAACTCC36250.11242119274388652Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGAGAAAGGCAGTTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA36190.11223511628693113Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGTCTGAACTCCA36070.11186296337302035Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA35950.11149081045910955Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGAAGGTAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC35770.11093258108824335Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGGAGATCGGAAGAGCACACGTCTGAACTCC35750.11087055560259154Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC35410.10981612234651095Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA34800.10792434503413108Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG34240.10618763143588068No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACACGTCTGAACT33180.1029002806963353Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAG32370.10038824852743743Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGTCTGAACTCCA32350.10032622304178565Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG1750.044.0185741
TTCTACG207.8422856E-444.018571
TTAGGGG302.5218123E-644.018571
TAAAGCT302.5218123E-644.018571
TTATGTC4850.044.018571
CTATACG900.044.018571
TATGGCA4850.044.018571
CTATACA207.8422856E-444.018571
GAATCGT650.044.018571
TGACCGA302.5218123E-644.018571
CCTCCGT454.783942E-1044.018571
AATGACA11750.044.018571
TATTACA750.044.018571
CGAATGT502.7284841E-1144.018571
GATTGCA5300.044.018571
TTCGGGT551.8189894E-1244.018571
CATCGCA1000.044.018571
AGAGGGG408.289135E-944.018571
TATAAAC408.289135E-944.018571
TAATGTA302.5218123E-644.018571
Produced by FastQC (version 0.11.5)