FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7627339
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA245541032.192223264234094Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA3399554.457059008390737Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC3099484.063645263439844Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1945522.5507191957771904Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC1748062.291834675238638Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1600242.09803182997373Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1453801.9060382657700148Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC1118791.4668156220668833Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG754510.9892178648411982Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC707580.9276891980283033Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA689270.903683447136675Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG550040.7211427209410779Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC520280.6821251815344774Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA405570.5317319710058777Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC359330.4711079447235792Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA343370.4501832159289104Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG332760.4362727289294471Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT326390.427921192436838Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC311090.40786177197578344Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC309420.4056722796770932Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC275470.36116134342527584Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC269690.35358334013998854Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA257300.33733914278623256Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA232920.30537517737181996Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG228280.29929179757186614Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC227010.29762673456627536Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA224040.2937328470650118Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG218160.28602373645644963TruSeq Adapter, Index 22 (97% over 37bp)
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACGTCTGAACTCC179100.2348132159852866Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA173510.22748431661422153Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACGTCTGAACTCC171740.22516371699225643Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC164760.21601242582767072Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG163330.2141375911048401Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA157150.20603515852645332Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC153630.20142018074717802Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA153230.20089575145407854Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG150020.1966872063769553No Hit
AAACCGTTACCATTACTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG149570.19609722342221841Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC145640.19094470561751614Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTC140730.1845073360447202RNA PCR Primer, Index 24 (100% over 34bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTAAACTCCA134690.1765884537189182Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC125000.16388415409358364Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA122350.16040981002679966Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC121370.15912495825870593Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA119690.1569223552276882Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA110810.14528002492088Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA108920.14280209651098502Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA108190.1418450130510785Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC106070.13906553779765132Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGACTCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC105010.13767580017093772Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA103660.13590585130672703Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA98320.12890472024384914Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGTCTGAACTCCA97490.12781652946066774Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC92360.12109072377666708Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC91640.12014675104908802Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCA87770.11507289763835067Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC87160.11427314296637399Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAG83120.10897640710606936Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC82220.10779644119659555Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA80580.10564628109488775Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC80250.10521362692808069Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACCGAGTAGATCGGAAGAGCACACGTCTGAACTCCA79980.10485963715523854Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCG79680.10446631518541394Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACGTCTGAACTCC78750.10324701707895768Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCA78490.10290613803844303Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGCTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA78340.10270947705353073Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTAGATCGGAAGAGCACACGTCTGAA78160.10247348387163596Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAACGGAATCCCAAAAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCA77530.1016475077350043Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTGCTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA76700.10055931695182291Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGTTG600.044.3300979
TAGAGTC254.2533422E-544.3300939
AGTCCTC254.2533422E-544.3300939
AAATATG502.5465852E-1144.3300939
ATATGCG551.8189894E-1244.33009311
TGCGCTG850.044.3300939
AGGCATT351.3649333E-744.33009310
GGCGGTT254.2533422E-544.33009311
CGGTGGT254.2533422E-544.3300939
GGCATTT351.3649333E-744.33009311
AAGGCGG254.2533422E-544.3300939
AAGGCAT351.3649333E-744.3300939
CATCATG454.456524E-1044.3300939
CTTACAC254.2533422E-544.33009310
CCATTAT3250.044.33009310
ATCCGAT1950.044.3300910
CTATTAC5700.044.3300910
CGTGAAT6600.044.3292166
GCAATTT750.044.3292167
CGATACC2650.044.3292165
Produced by FastQC (version 0.11.5)