FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7776386
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG98039912.607386001672241No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT5466317.029370712822127No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC4277215.500254231207144No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG3974675.111204613556992No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT3828034.922633727286685No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC3051923.924599421890837No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC2055342.643052955447428No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC2052062.6388350578276336No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA1327671.7073097966073185No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1240401.595085429144078No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG814811.0478003535318334No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG715490.9200803560934346No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG688510.8853855762818358No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC604240.7770190420074312No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT569480.7323196147927843No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA545820.7018941703768306No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC543000.6982678071793247No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT538820.6928925596028799No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC465580.5987099920194291No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT388750.49991088405333783No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG381040.48999625275802927No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT372400.4788856931741814No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA357650.4599180133290709No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG356510.4584520367173131No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG341400.43902141688954227No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT305100.39234163530462607No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG260640.3351685474460758No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG208340.26791365552070073No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG208220.2677593421931473No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACTAGATCGGA205350.2640686817758275No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA202350.26021084858699145No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC201320.2588863258588244No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC193170.24840587902915315No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGT183970.23657519058338924No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG173310.22286702331905847No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG172340.22161965725466817No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT169320.21773610517790654No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC163770.21059911377855986No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGC163230.20990470380456938No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT161970.20828441386525823No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT158900.20433656456868265No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG152400.19597792599287123No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG151650.19501346769566225No Hit
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGT141230.18161392708643834No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT140420.1805723121254526Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT135550.17430976291557543No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC134740.1732681479545897No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG129190.16613115655524302No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT129170.16610543766731745No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTC128490.16523099547784795TruSeq Adapter, Index 20 (97% over 40bp)
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC124850.16055015787539353No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG120920.15549639639801832Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT117550.15116276378255916Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG113090.14542745177515623No Hit
TGTAAACATCCCCGACTGGAAGATCGGAAGAGCACACGTC112850.14511882512004934No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCC111970.1439871940513241TruSeq Adapter, Index 20 (100% over 39bp)
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT110330.14187824524142706No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC107990.13886913535413495No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC107540.13829046037580953No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC106940.13751889373804232No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG105280.1353842260402197No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAG104700.13463837829037806No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG103270.13279947780369955No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACG101240.13018901067925384No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT99220.1275914029987709No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACG98990.1272956357876268No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG94790.12189466932325632No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG94230.12117454046134027No Hit
TATTGCACTTGTCCCGGCCTGTCAGATCGGAAGAGCACAC93450.12017150383224288No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT90970.11698236172947177No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT90880.11686662673380668No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG90070.11582501177282095No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC85800.11033402920071096No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA83790.10774928096419081No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT83580.1074792326409723No Hit
CAAAGAATTCTCCTTTTGGGCTAGATCGGAAGAGCACACG81180.10439296608990346No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACAGATCGGAA80510.10353138334439674No Hit
TATTGCACTTGTCCCGGCCTAAGATCGGAAGAGCACACGT79100.10171820174564379No Hit
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACG78940.10151245064223921No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)