FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5489328
Sequences flagged as poor quality0
Sequence length40
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG4714348.588191487191146No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT3672136.6895802181979285No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT3322356.052380182055071No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC2392044.357619001815887No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC2273344.141381240108079No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1278232.328572823485862No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC1207962.200560797241484No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC696941.26962717476529No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA690761.2583689661102415No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG650191.1844619232080866No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG555341.0116721026690334No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT522570.951974449331503No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT517720.943139123768884No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG511830.9324092129309817No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG474630.8646413550073889No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT419890.7649205877294999Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT389260.7091214079391868No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA381990.6958775281783125No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC344100.6268526857932337No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC310740.5660802196553021No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA235080.4282491408784463No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG225150.4101594949327131No Hit
AAACCGTTACCCTTACTGAGTAGATCGGAAGAGCACACGT216090.3936547424384187No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC210310.3831252204277099TruSeq Adapter, Index 7 (100% over 40bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC208480.3797914790298557No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT197870.360463065788745No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG194040.3534858911691923No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG191670.3491684228014795No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC187450.34148077870369564TruSeq Adapter, Index 7 (100% over 39bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG178080.324411294060038No Hit
GGCTGGTCCGATGGTCGTGGGTTATCAGAACTAGATCGGA170120.3099104298376778No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC167640.30539257264277153No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT158490.2887238656535008No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG153460.27956063110092894Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG150360.2739133096072962No Hit
TCCGGGTGCCCCCTCCAAGATCGGAAGAGCACACGTCTGA150130.27349431478680086Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT147390.26850281127307385No Hit
TCCCCGGCATCTCCACCAAGATCGGAAGAGCACACGTCTG145640.2653148072040876Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT144750.2636934794204318No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG143980.26229075763007786No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT143050.26059656118198804No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG123830.22558316792146504No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG119880.21838738730861046No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG118280.2154726407312516No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT115560.2105175715497416No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC114790.20911484975938768No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG111000.20221054380426895No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACG108950.19847602475202794No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT107100.1951058490219568Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC106290.1936302585671689No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT102750.18718138176476246No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA102080.18596083163549346No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC101810.18546896815056416No Hit
ACCGGGCGGAAACACCAAGATCGGAAGAGCACACGTCTGA101410.18474028150622443Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA93770.170822366599336Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC92040.16767079686256678No Hit
CAAAGAATTCTCCTTTTGGGCTAGATCGGAAGAGCACACG91950.16750684236759034No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT90890.1655758227600901No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC89080.16227851569445295No Hit
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACG87970.16025641025641024No Hit
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGT86980.15845291081166948No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG86140.1569226688585561No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG85960.15659475986860322No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA83730.15253233182640935No Hit
AAACCGTTACCATTACTGAGTTTAGATCGGAAGAGCACAC81120.14777765147209276No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG79680.1451543795524698No Hit
TGTAAACATCCCCGACTGGAAGATCGGAAGAGCACACGTC78450.14291366812112521No Hit
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGT75170.1369384376375396No Hit
TTATGTCTTGTTGATCTCAATAGATCGGAAGAGCACACGT71980.13112716164893043No Hit
CAACGGAATCCCAAACGCAGCTAGATCGGAAGAGCACACG71570.13038025783848223No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG71060.1294511823669491No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT70610.12863140989206695No Hit
TGGAGAGAAAGGCCGTTCCTGAAGATCGGAAGAGCACACG70260.12799380907826968No Hit
GGGATTGTGGGTAGATCGGAAGAGCACACGTCTGAACTCC70020.12755659709166586Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACA67790.123494169049472No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG67780.12347595188336351No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG67580.12311160856119366No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCA66610.12134454344866986No Hit
TCCCCGGCACCTCCACCAAGATCGGAAGAGCACACGTCTG66190.12057942247211317Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTC65840.11994182165831592No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT65820.11990538732609894No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC65460.1192495693461932No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC63620.11589761078223054No Hit
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT62310.113511162022018No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC61580.11218130889609802No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT60410.11004990046140438No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT59530.10844678984385703No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGC58980.10744484570788992No Hit
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGT57800.10529522010708778No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT56760.10340063483180455No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG56090.10218008470253555No Hit
GGCTGGTCCGATGGTCGTGGGTTATCAGAACAGATCGGAA55870.1017793070481487No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT55040.1002672822611438No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAG55030.10024906509503531No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC54990.10017619643060134No Hit
TGGAGGCGTGGGTAGATCGGAAGAGCACACGTCTGAACTC54940.10008511060005888Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATATA301.4838974E-534.0026971
TTGGCGG200.002788815434.0026971
AAATCGT1850.034.0026971
CTCGTAC3450.034.0026971
CAACGGG504.456524E-1034.0026971
TAGAATG200.002788815434.0026971
CATTGCG455.989932E-934.0026971
CTATACG750.034.0026971
TTATGCC252.0281719E-434.0026971
TTCAAGT6050.034.0026971
AATGGCG5650.034.0026971
CGACATG553.274181E-1134.0026971
TCCTATA1750.034.0026971
AAAGTTC455.989932E-934.0026971
TATTGCG1400.034.0026971
AAGCCGT2050.034.0026971
TCATGTT252.0281719E-434.0026971
TCATGTA750.034.0026971
TATTACA850.034.0026971
AAACAGT351.0916701E-634.0026971
Produced by FastQC (version 0.11.5)