FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8270902
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG136550216.509710790914944No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT6599197.978803279255395No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC5661476.845045437607651No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG3497914.229175971375311No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC3414094.1278327321494075No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC3363164.066255409627631No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT2787763.3705634524505306No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC2142582.590503429976561No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1520571.8384572807173871No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA1149481.3897879578309595No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC812460.9823112400558004No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG791300.9567275733650331No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT651120.7872418268285611No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG626840.7578858992646751No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG583880.7059447687809625No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC508370.6146488013036547No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC475240.5745927106862105No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA426770.5159896707759323No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG413310.49971575047074673No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT377420.456322659850159No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA363010.4389001344714276No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG324470.39230304022463325No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG309710.3744573445556482No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT307160.3713742467266569No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG298130.36045645323811115No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT295320.3570590003363599No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC285090.34469033728123005No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT273800.3310400727756175No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG226400.27373072489554345No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG202490.24482214878135422No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT195150.23594766326574781No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC183380.22171705093350155No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT172380.20841741323981353No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACTAGATCGGA170370.20598720671578505No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA163060.19714899293934326No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC160690.19428352554533956No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC153270.18531231539196089No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG147530.17837232263180003No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT145030.17534967770141638No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC144130.17426152552647825No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT141230.1707552574072332Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG141080.17057389871141018No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT138270.16717644580965899No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG132820.16058707986142262No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG129650.15675436608969615No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG128080.1548561450734152No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTC121260.1466103697033286TruSeq Adapter, Index 11 (100% over 40bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA120990.14628392405084717No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG117790.1424149385399561No Hit
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGT117490.14205222114831006No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAG116090.14035953998729522No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC115310.13941647476901553No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT110800.1339636233146034No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG110380.13345581896629896No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC110220.13326236969075442No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG105360.12738634794608858No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT105360.12738634794608858Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACG98280.11882621750324208No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC97450.11782269938635472TruSeq Adapter, Index 11 (100% over 39bp)
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT97250.11758088779192404No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT95760.11577939141341537No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC95340.11527158706511091No Hit
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGT91760.11094315952480154No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA89820.1085975870588238Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT88850.10742480082583496No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG87900.10627619575228917No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG85270.10309637328552557Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC84790.10251602545889192No Hit
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT84300.10192358705253671No Hit
TGTAAACATCCCCGACTGGAAGATCGGAAGAGCACACGTC84100.10168177545810601No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA252.0277896E-434.004042
AGGTACT301.4835863E-534.004043
ACAGTAC301.4835863E-534.004042
ATCAGTT301.4835863E-534.004041
AGCTTAT1400.034.004042
CAACCGT351.0914209E-634.004041
GTCTACG1200.034.004041
TAACGGA900.034.004041
ATGTAGT750.034.004043
TCAATCA301.4835863E-534.0040427
AGTGCAT3400.034.004043
AATGACA23450.034.004041
TAGGGGC252.0277896E-434.004042
CGGTGCG601.8189894E-1234.004041
GATGCCT750.034.004041
AATTACA301.4835863E-534.004041
ACCGTCA750.034.004043
GCAGCAT12750.034.004042
TTGCAAT301.4835863E-534.004043
CGGTACT14000.034.004043
Produced by FastQC (version 0.11.5)