FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7902400
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG108010913.668113484511036No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT5364096.787925187284875No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC5100036.453773537153269No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC4025085.093490585138692No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT3345524.233549301478032No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG2810973.5571092326381857No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC2728853.4531914355132614No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1803042.2816359586960924No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1101951.3944497874063575No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG852081.078254707430654No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA830411.050832658432881No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG796551.0079849159748937No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC665840.8425794695282447No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT621670.7866850577039887No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC592140.7493166632921644No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG479490.6067650334075724No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC459690.5817093541202673No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT455730.5766982182628062No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA441660.5588935007086454No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT440210.5570586151042721No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG435660.5513008706215833No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG415470.5257516703786191No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG360770.45653219275156914No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG293470.3713681919416886No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT259620.3285331038671796No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA255540.32337011540797733No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC242970.3074635553755821No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG241390.3054641627859891No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC222760.28188904636566103No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT220790.279396132820409No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT216630.2741319092933793No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC197440.24984814739825875No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT191650.24252125936424376Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG189300.2395474792468111No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT184970.2340681312006479No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC182770.23128416683539177No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG180820.22881656205709658No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT176320.22312208949179993No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT171410.21690878720388743No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG164410.20805071876898157No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG161890.20486181413241544No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG158310.20033154484713506No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT146860.18584227576432477No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT145430.1840326989269083No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG142310.18008453128163596No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC141430.1789709455355335TruSeq Adapter, Index 7 (100% over 40bp)
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC140090.17727525814942297No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC133660.16913848957278801No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG133560.16901194573800365No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC128210.16224185057703988No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC127610.16148258756833367TruSeq Adapter, Index 7 (100% over 39bp)
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA126580.16017918607005466No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC126300.15982486333265844No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACTAGATCGGA117490.1486763514881555No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA114510.1449053452115813No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG114430.1448041101437538No Hit
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT107750.1363509819801579No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT106700.13502227171492207No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACG105710.1337694877505568No Hit
CAAAGAATTCTCCTTTTGGGCTAGATCGGAAGAGCACACG105110.13301022474185056No Hit
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACG100210.12680957683741648No Hit
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGT98470.12460771411216846No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG98220.12429135452520752No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC97340.12317776877910508No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG90260.11421846527637172Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT89590.11337062158331647No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT89470.11321876898157522Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC89420.11315549706418303No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG86630.10962492407369911No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG85060.10763818586758453No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC84790.10729651751366673No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG84040.10634743875278396No Hit
ATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACG82370.10423415671188499No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT81790.10350020247013565No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA80930.1024119254909901Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA80310.10162735371532698No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTA252.0274012E-434.0051271
AAATCGT1650.034.0051271
ATCAGTT200.002787911834.0051271
CTATACG1650.034.0051271
GTCTACG1650.034.0051271
CGACATG200.002787911834.0051271
ACCCTAT200.002787911834.0051271
TATGACA200.002787911834.0051271
TATTGCG2850.034.0051271
AAGCCGT2150.034.0051271
TCATGTA1450.034.0051271
TATTACA800.034.0051271
TCGTATC408.065217E-834.0051271
TCGAAAC2900.034.0051271
AATGTGT200.002787911834.0051271
TCCTATA2650.034.0051231
ATCACAT1200.034.0051231
AATTACA601.8189894E-1234.0051231
AACCGTG504.456524E-1034.004052
AGTCCGG504.456524E-1034.004052
Produced by FastQC (version 0.11.5)