FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4463569
Sequences flagged as poor quality0
Sequence length40
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG3589088.040830107028702No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT3428017.679975373966438No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT2750946.1630950479313755No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC2199764.928253601546206No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC2025334.537467663208522No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA1904004.265644823682573No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1164072.6079354883950487No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT941922.1102395863041434TruSeq Adapter, Index 10 (100% over 39bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC940612.1073047151281856No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG701801.5722844208300577No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT668501.4976804436091389No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG509151.1406791291901168No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG467961.04839871412316No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC456761.0233066857485569No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA364700.8170591739480223No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT363940.8153565005940314No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC331120.7418278960177382No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT306710.6871407163191607No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG298100.6678512195061843No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA269260.6032392464415807No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT267950.6003043752656226No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG267730.5998114961368358No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG230150.5156187795013363No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT209580.46953458095976564No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG178080.3989632511561936No Hit
AGATCGGAAGAGCCCACGTCTGAACTCCAGTCACTAGCTT159710.3578078439024915TruSeq Adapter, Index 10 (97% over 39bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT155780.3490032303746172No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT153210.3432455060065163No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC134390.3010819368984774No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC133810.2997825282862212No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC133640.29940166714124955No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG131160.29384557514401594No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA121290.2717332251388967No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG120810.2706578524942709No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA118930.2664459763028195No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA115460.2586719282260451No Hit
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT112770.2526453606967877No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC102720.23012974594993377No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA101590.2275981395157104No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG100090.2242376000012546No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG98100.2197792842454099No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG97540.21852468282667972No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG96040.21516414331222392No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT94800.21238609731360714No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGG94190.21101947791106176No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC94160.21095226712077264No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG93370.20918238297649255No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT92630.20752451681602774No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT92500.20723327005810818No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC92220.20660596934874315No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG91600.20521694634943471No Hit
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACG91510.20501531397856737No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA89980.20158756367382244No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC87960.1970620371276886No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG86190.19309660050063077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGT84520.1893551998412033No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC83220.18644273226200828No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG80110.17947521366870323No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG80050.179340792088125No Hit
TCGCTCTGATACCCAATTGATGAGATCGGAAGAGCACACG77410.17342624254268277No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC76940.17237327349481996No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG75150.16836302967423603No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATC72900.1633222204025523No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG72810.16312058803168494No Hit
CTAGACTGAAGCTCCTTGAGAAGATCGGAAGAGCACACGT70540.15803497156647517No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT69650.1560410514545647No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGA63750.14282292936437188No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACAC58190.13036652956412234No Hit
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGT57200.1281485734845815No Hit
TTGAATTGAAGTGCTTGAATCAGATCGGAAGAGCACACGT54740.12263728868087398No Hit
TCCTGTACTGAGCTGCCCCGAGTTAGATCGGAAGAGCACA54660.12245805990676968No Hit
CTAGACTGAAGCTCCTTGAGGAAGATCGGAAGAGCACACG54240.12151710884272206No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT54050.1210914405042243No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC52530.11768609379624241No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG52350.11728282905450772No Hit
CCCCCCACTGCTAAATTTGACTGGTAGATCGGAAGAGCAC49980.11197317662166754No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA49430.11074097879970042No Hit
TCTCTCGGCTCCTCGCGGCTCGTTAGATCGGAAGAGCACA48690.10908311263923556No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA47100.10552094075391238Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACAC46800.10484883285102123No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG46710.10464720048015388No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAGT700.034.0050771
AATCCTT200.002787803334.0050771
AAATCGT1100.034.0050771
TTAGACT252.0272774E-434.0050771
CTATACG2700.034.0050771
CTATACA408.063944E-834.0050771
TCGGGTG301.4831243E-534.0050771
GTCTACG1200.034.0050771
TCAGCCG301.4831243E-534.0050771
AAACCGC1550.034.0050771
AGTCTTG200.002787803334.0050771
TATCGCA1000.034.0050771
TCAGTGC11500.034.0050771
GCGCAGT1750.034.0050741
TATTGCG1050.034.0050741
AAGCCGT1450.034.0050741
AGAGGTA900.034.0050741
TTGATAT351.0915846E-634.002793
ACCGGTA252.0280782E-434.002793
CAGTTTC553.274181E-1134.002793
Produced by FastQC (version 0.11.5)