FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5511668
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG87077815.798810813713743No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT5124709.297911267514662No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC3685686.6870500908255No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC3207575.819599438863154No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1983343.5984388029177374No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT1766753.2054724631454583No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC1627572.9529536249280617No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1004341.8222069979541584No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC971761.7630960355376992No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG533290.9675655355148386No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC470920.8544055991761477No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC402000.7293617830391815No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG380590.6905169179275675No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC376430.6829692935060675No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG348710.6326759884666493No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA332610.6034652304892095No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT316600.5744177624631963No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT295140.5354821807119006No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC285270.5175747160387745No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT259800.4713636597850233No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG257770.4676805642139548No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG250880.4551798112658455No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT246170.44663430380784913No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG232770.42232224437320964No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT229880.4170788225996196No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT216340.392512756573872Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT184920.33550642019802357No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT158990.2884607708592027No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG156450.28385236556338295No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC147790.2681402435705489No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT139180.25251883821739624No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA138510.2513032352456643No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC133470.2421589979657701No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACG130040.23593583648361982TruSeq Adapter, Index 22 (100% over 39bp)
TATTGCACTTGTCCCGGCCTGGAGATCGGAAGAGCACACG129340.23466580352807898No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT117560.21329296321911986No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG111620.20251582642495883No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT110460.20041120038434826No Hit
AAACCGTTACCCTTACTGAGTAGATCGGAAGAGCACACGT109940.19946774733166076No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC103520.18781973079655742No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT101560.184263638521043No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT100910.18308432220518364No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC100790.18286660226994803No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG97870.17756875051254903No Hit
TCCTGTACTGAGCGTCGCTAGATCGGAAGAGCACACGTCT97680.17722402728175937Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC96660.17537340783225694No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG92340.1675354901637762No Hit
CAAAGAATTCTCCTTTTGGGCTAGATCGGAAGAGCACACG91730.166428747159662No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC89290.1620017751432053No Hit
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGT88760.16104017876258148No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTC80340.14576349664021854TruSeq Adapter, Index 22 (97% over 40bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG80250.14560020668879184Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT79020.1433685773526272No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACG78120.14173567783836036No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG73760.1338251868581344No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGC73590.13351675028321736No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC72950.13235557729529426No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG72920.13230114731148537No Hit
TCCTGTACTGAGCTGCCCCGGGAGATCGGAAGAGCACACG70430.12778345865534715No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC68390.12408221975634237No Hit
TGTAAACATCCCCGACTGGAAGATCGGAAGAGCACACGTC68360.12402778977253348No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG67840.12308433671984598No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG67410.12230417361858514No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG66780.12116114395859838No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC61980.11245234654917531No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT61620.11179918674346857Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA61600.11176290008759598Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT60840.11038400716443734No Hit
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACA60390.10956755740730392No Hit
CAACGGAATCCCAAACGCAGCTAGATCGGAAGAGCACACG59150.10731778474320297No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT57530.10437856561752268No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG56260.10207436296961284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)