FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8088958
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG137057216.94373984881613No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT6123837.57060427313382No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC5044946.236823086484069No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC3864344.777302589530073No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC3377064.174901143014959No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA2017722.494412753781142No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1804302.230571601434944No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1515261.8732449840881855No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1294921.600848959779492No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT1231061.5219018321024784No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT915261.1314930798256093No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC775560.9587885114498059No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA649640.8031195118085668No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG543580.6720025001984187No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG500510.6187570760041033No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC471750.5832024347264505No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG429800.5313416140867588No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT414510.5124393030598008No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG409750.506554737952651No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC396900.4906688846696942No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG390290.48249725119106807No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC301660.3729281323997479No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG299650.3704432635204683No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACTAGATCGGA293040.3622716300418422No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT270510.33441884603678246No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA269890.3336523690690445No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG262170.32410849456753266No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT255260.31556598513677536No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC250960.3102500964895602No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA249120.30797539064982166No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC247160.3055523344292306No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT233390.2885291282264045Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG185760.2296463895596936No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT184190.22770547207687317No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG173880.21495970185529456No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG172650.2134391104515563No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT172630.21341438538808088No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG171820.2124130203173264No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA167530.207109494201849Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT166930.20636774229758642No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG166280.2055641777346353No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC165430.20451336253693No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG160930.19895022325496065No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT157980.1953032763923363No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT151040.18672367936636583No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG148180.1831879952893809No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT145890.1803569755214454No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC145470.1798377491884616No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC139910.17296418154229506No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC138160.17080073848819588No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT130870.16178845285140558No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG130800.16170191512924162No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC127430.15753574193363348No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC126370.15622531356943625TruSeq Adapter, Index 4 (100% over 40bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAG126020.15579262495861643No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA119630.14789296717821998No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA119360.14755917882130185TruSeq Adapter, Index 4 (100% over 39bp)
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG117670.1454699109576289No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT117590.14537101070372724No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCA116920.14454272107730068No Hit
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGT115400.1426636162531688No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT112570.139165019771397No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGT107760.1332186420055587No Hit
CCCCCCACTGCTAAATTTGACTGGAGATCGGAAGAGCACA107390.13276122833126344No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT105420.13032580957893464No Hit
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGT103150.12751951487447458No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACAC101280.12520772143952286No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT96780.11964458215755354No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC96100.1188039299993893No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT95410.11795091530948731No Hit
TATTGCACTTGTCCCGGCCTGGAGATCGGAAGAGCACACG93270.11530533351761749No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAG91160.11269683932096075No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA90640.11205398767059985No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACAGATCGGAA89530.11068174664771407No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG88780.10975455676738585No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA87100.10767765143545065No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGC87030.10759111371328668No Hit
TCAGGCTCAGTCCCCTCCCGAAGATCGGAAGAGCACACGT86400.10681227421381098No Hit
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACG85340.10550184584961375No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACG83670.10343730304941626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTA700.034.003993
CTACGGC1000.034.003993
TTAACAT252.027804E-434.003993
CTGTATT5200.034.0039863
CTGTACG950.034.0039863
CAAGTCA200.002788373734.0039863
TAAACCT4350.034.0039863
TACATGA200.002788373734.0039862
ATTCCTT200.002788373734.0039862
AGTGCAT1500.034.0039863
CCATATG900.034.0039863
AAGGGGC408.067764E-834.0039862
CGGTACT13700.034.0039863
CCTATAC4300.034.0039862
CCCATAT900.034.0039862
TACACAT750.034.0039863
TAACTAA200.002788373734.0039863
GGTACTA200.002788458334.0037774
TCGTTAC700.034.0037774
ACATTGC700.034.0037774
Produced by FastQC (version 0.11.5)