FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6434916
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG86916013.506936221078877No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT5308418.24938507355807No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC3447785.357925418140656No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT3002794.666401239736462No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC2507933.897377992191351No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC2108373.2764530259602456No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1362442.117261515146429No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1205051.8726740178115768No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA1124251.7471090531717897No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT1075551.6714281895832053No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG941191.462629815214371No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT603500.9378521801993996No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG575250.8939510632306622No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC567100.8812857852379113No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG523820.814027720019966No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG495230.7695982356257642No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT424740.6600552361522668No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT389660.6055401500190523Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA339390.5274194721422937No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC321130.4990430333511735No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG298090.463238370166759No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG296120.4601769471427443No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC237500.3690801869053147No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG236240.367122119387417No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT232960.3620249277535247No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA214910.33397483354872076No Hit
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC209460.32550541452289355No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTC207530.32250615237246294TruSeq Adapter, Index 3 (100% over 40bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG206220.32047038376258524No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC184000.2859400184866438No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC179930.2796151495994664TruSeq Adapter, Index 3 (100% over 39bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACG175500.2727308328500325No Hit
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGT174070.2705085816194026No Hit
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGT169220.26297157569733626No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA168850.2623965876166837No Hit
GGCTGGTCCGATGGTAGTGGGTTATCCGAACTAGATCGGA168380.26166619735207114No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG167880.26088918643227044No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT156080.24255172872497482No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT140090.21770291950974963No Hit
TGAGGGGCAGAGCGCGAGACTAGATCGGAAGAGCACACGT140010.21757859776258154No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG135500.21056995926597954No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC135240.2101659135876832No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT133910.20809906454101343No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT133510.2074774558051729No Hit
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGT124290.1931493744440487No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT123400.1917662950068035No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG122820.19086496233983474No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG122790.1908183416846467Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT121980.18955958399456962No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG114260.17756253539284741No Hit
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACG112450.174749755863169No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC112210.1743767906216647No Hit
TCCTGTACTGAGCTGCCCCCAGATCGGAAGAGCACACGTC110650.17195251655188662No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT109250.16977688597644477No Hit
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTC105480.16391822364114778No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCA101370.15753119388038633No Hit
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACG99480.15459409260353982No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT97030.15078673909651655Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACAC91760.14259704400181758No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA91060.14150922871409666Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG89900.1397065633801591No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC89170.13857212743725017No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC81430.1265439983987359No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG79960.12425958629452194No Hit
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGT77540.12049885344268675No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGT75600.11748405107386017No Hit
TCTCTCGGCTCCTCGCGGCTCAAGATCGGAAGAGCACACG74740.11614759229180303No Hit
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACG72780.11310170948618443No Hit
TTATGTCTTGTTGATCTCAATAGATCGGAAGAGCACACGT72530.11271320402628411No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACG72100.11204497463525555No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG70320.10927881576076517No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT69850.10854842549615255No Hit
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGT67930.10556470356411801No Hit
CATGCCTTGAGTGTAGGACCGTAGATCGGAAGAGCACACG67570.10500525570186153No Hit
TGAGGTAGTAGTTTGTGCTGTAGATCGGAAGAGCACACGT66650.10357555560942833No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGC65290.1014620859075705No Hit
TTGAATTGAAGTGCTTGAATCAGATCGGAAGAGCACACGT64710.10056075324060175No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATATA200.00278774134.005461
AAATCGT950.034.005461
GGATGTA455.984475E-934.005463
AGATAGT200.00278774134.005463
TTGCTTC408.064126E-834.005462
TTATGCC200.00278774134.005461
AGGAATG200.00278774134.005462
CGACATG800.034.005461
TAGCCTG200.00278774134.005462
CCATCGA200.00278774134.005462
AGGTTCA200.00278774134.005463
GATATAG200.00278774134.005462
CGTGTAC408.064126E-834.005462
AATTACA200.00278774134.005461
TTTGCTT408.064126E-834.005461
AGCTACA950.034.005461
AAAGCCG200.00278774134.005462
TCAGTGC12850.034.005461
CAACGGG301.483113E-534.0054551
ACCATCG252.027244E-434.0054551
Produced by FastQC (version 0.11.5)