FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5082561
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA62843412.364514661014397Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA2126674.184248846201748Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC1864363.668150761004147Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1155612.2736765972902244Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTC1118252.2001703471930782TruSeq Adapter, Index 11 (100% over 50bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC1071562.108307209692122Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC1069022.1033097290912983Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA1028372.023330364357654Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC966051.900715013553207Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG767171.509416217532854Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC759101.4935383953089791Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG609691.1995724202818225TruSeq Adapter, Index 11 (100% over 49bp)
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC596681.173975088542961Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA590531.161874889450417Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACGTCTGAACTCC537781.058088628941197Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA448550.8825275289366916Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC424370.8349530876264938Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACGTCTGAACTCC415980.818445661547397Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATTTGGTGTTTCTTCGATCAGATCGGAAGAGCACACGTCTGAACTCCA402990.7928876800494868Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC302650.5954675211964991Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC262110.5157045827880865Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGACATGGGACTGCCTAAGCTAGATCGGAAGAGCACACGTCTGAACTCCA259050.509683995922528Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG257270.5061818244778568Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG251960.49573433550526985No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC225660.4439887686542277Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC188580.3710334219304008Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTAGCAGCTGTTGAGCAGGTAGATCGGAAGAGCACACGTCTGAACTCCA174670.3436653293487279Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG167440.3294402172448102Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTGTTCTCAGGTCACCCCTTAGATCGGAAGAGCACACGTCTGAACTCCA161470.3176941703208284Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTATGTCTTGTTGATCTCAATAGATCGGAAGAGCACACGTCTGAACTCCA144480.2842661406326456Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAGATCCGGACTACAACAAAAGATCGGAAGAGCACACGTCTGAACTCCA143380.28210187738032066Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC136250.2680735164811598Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGTCTGAACTCCA134450.26453199479553713Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCAGTTTCTTGTTCGTTTCAAGATCGGAAGAGCACACGTCTGAACTCCA131660.259042636182822Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA130180.2561307183524211Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC129640.25506826184673437Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCATGGTCAGATCCGTCATCCAGATCGGAAGAGCACACGTCTGAACTCCA126340.2485754720897595Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG125360.24664731028314268Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA123840.24365669197083914Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC120060.23621949643103154Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAAGATCCGGACTACAACAAAGAGATCGGAAGAGCACACGTCTGAACTCC115030.22632291083176376Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATTGGAAATTTCGTTGACTAGATCGGAAGAGCACACGTCTGAACTCCA111210.21880701481005344Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA109280.21500971655824694TruSeq Adapter, Index 11 (100% over 40bp)
TCCCAAATGTAGACAAAGCAAGATCGGAAGAGCACACGTCTGAACTCCAG109080.21461621414873328Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCTGCTATGTTGCTGCTCATAGATCGGAAGAGCACACGTCTGAACTCCA104380.205368907525163Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTTCGTGAATATCTGGCATAGATCGGAAGAGCACACGTCTGAACTCCA98170.19315065770976483Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAACTAATCACGGAAATGCAAGATCGGAAGAGCACACGTCTGAACTCCA96730.1903174403612667Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC94940.1867955937961197Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTCCGGTTTTGGATACGTGAGATCGGAAGAGCACACGTCTGAACTCCA94840.18659884259136292Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGATCTCTTCGTACTCTTCTTAAGATCGGAAGAGCACACGTCTGAACTCC94840.18659884259136292Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA94260.18545768560377338Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACGTCTGAACTCC88290.17371163867979156Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA87960.17306235970409406Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC86870.17091777157224478Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCATGGTCAGATCCGTCATCTAGATCGGAAGAGCACACGTCTGAACTCCA85940.1690879853680064Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA85630.1684780566332603Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG84940.16712047332043825Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAAGATCCGGACTACAACAAAGATCGGAAGAGCACACGTCTGAACTCCAG80060.1575190145283057Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAATTTGGTGTTTCTTCGATTAGATCGGAAGAGCACACGTCTGAACTCCA79040.1555121522397862Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGCAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG77800.1530724373008017TruSeq Adapter, Index 11 (97% over 49bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC77010.15151810278322286Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTTTGTTGTACTCGGTCTAGCAGATCGGAAGAGCACACGTCTGAACTCC70080.13788324429357562Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGGCTCAGTCCCCTCCCGATAGATCGGAAGAGCACACGTCTGAACTCC66200.1302492975490112Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT65040.12796698357383215Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC64770.12743575532098878Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC64510.12692420218862105Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA63860.12564531935770176Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGAAACTGGTGTCGACCGACAAGATCGGAAGAGCACACGTCTGAACTCCA63050.12405163459917157Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACGTCTGAACTC61940.12186769622637092Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC61760.12151354405780866Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAATTGAAGTGCTTGAATCAGATCGGAAGAGCACACGTCTGAACTCCA61570.1211397167687707Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATGGATCTGTCTTCAAGGACAGATCGGAAGAGCACACGTCTGAACTCCA60410.11885740279359165Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGTCTGAACTCCA59040.1161619112884233Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA57780.11368284610848743Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC56880.11191208526567611Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA56830.11181370966329769Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC56720.1115972833380652Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGCCTCTGTGGTAGCCCTCAGATCGGAAGAGCACACGTCTGAACTCCA56370.11090865412141636Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGATCTCTTCGTCCTCTTCTTGAGATCGGAAGAGCACACGTCTGAACTCC53980.10620630032772847Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT53200.10467164093062532No Hit
CGAAACTGGTGTCGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC53200.10467164093062532Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA52680.1036485346658899Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAG52500.10329438249732763Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC52140.10258607816020308Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA51680.10168102261832175Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGTCTGAACTCCA51260.10085466755834313Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAG50910.10016603834169428Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATT207.834449E-444.0279961
CTATACG2100.044.0279961
TGCGGTA502.7284841E-1144.0279961
GCAGGCT950.044.0279961
TCATATG254.4283388E-544.0279961
TAAGCGT207.834449E-444.0279961
AAACCGT876950.044.012941
ACACGGG454.802132E-1044.006343
TATACGC302.5269292E-644.0063362
GCCGTAC207.8534574E-444.0063362
ATCGATA600.044.0063362
TCGTTAC17000.044.0041734
CCGCTAC2800.044.0041734
GGCACGT408.314601E-944.004174
CAGCACG650.044.004174
CCGTTAT800.044.004174
ATTCGCG302.5293248E-644.000285
TAACGTA454.802132E-1044.000285
CCCTTCG351.4473153E-744.000285
TCGCCTT207.858786E-444.0002755