FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727791
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG83547148.3548646798137TruSeq Adapter, Index 9 (100% over 49bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC1348247.803258611718662TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCCCACGTCTGAACTCCAGTCACGATCAGATCTCGTATG703854.0736987286078TruSeq Adapter, Index 9 (97% over 49bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG636943.686441242025222TruSeq Adapter, Index 9 (97% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG383842.2215649925251375TruSeq Adapter, Index 9 (100% over 49bp)
AGATCGGCAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG351942.0369361803597776TruSeq Adapter, Index 9 (97% over 49bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG295591.7107971971146976TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACCCGTCTGAACTCCAGTCACGATCAGATCTCGTATG211651.2249745484262853TruSeq Adapter, Index 9 (97% over 49bp)
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG125810.7281551993267704TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGCAGAGCCCACGTCTGAACTCCAGTCACGATCAGATCTCGTATG122380.7083032612162004TruSeq Adapter, Index 9 (100% over 36bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG112180.6492683432197529TruSeq Adapter, Index 9 (97% over 49bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG74740.43257546775043976TruSeq Adapter, Index 9 (100% over 49bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG74740.43257546775043976TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCCCCCGTCTGAACTCCAGTCACGATCAGATCTCGTATG72190.41781673825132787TruSeq Adapter, Index 9 (100% over 34bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG55220.319598840369003TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGCAGAGCCCCCGTCTGAACTCCAGTCACGATCAGATCTCGTATG54470.31525803757514653TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACGATCAGATCTCGTATG51760.2995732701466786TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGATCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG49020.28371487060645645TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGCAGAGCACCCGTCTGAACTCCAGTCACGATCAGATCTCGTATG47470.27474387816581985TruSeq Adapter, Index 9 (100% over 34bp)
TGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC44070.255065572167004TruSeq Adapter, Index 9 (98% over 50bp)
TGAGCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG35660.2063907035052272TruSeq Adapter, Index 9 (97% over 49bp)
GGATGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC33740.19527824835295474TruSeq Adapter, Index 9 (98% over 50bp)
ATAGCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG32950.19070593607675929TruSeq Adapter, Index 9 (100% over 46bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC32170.1861915011711486Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG28460.16471899668420545Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCGGTCACGATCAGATCTCGTATG27320.15812097643754366TruSeq Adapter, Index 9 (97% over 49bp)
TTATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG24310.14069988789153318TruSeq Adapter, Index 9 (97% over 49bp)
AAACCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG23920.13844267043872782TruSeq Adapter, Index 9 (100% over 46bp)
AGATCGGAATATCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG23810.13780601936229556TruSeq Adapter, Index 9 (100% over 38bp)
GGAGCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG21300.12327879934552269TruSeq Adapter, Index 9 (97% over 49bp)
AGAGCGGAAGATCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG21020.12165823296914963TruSeq Adapter, Index 9 (100% over 38bp)
AGATCGGAAGAGCACGCGTCTGAACTCCAGTCACGATCAGATCTCGTATG20920.1210794592633021TruSeq Adapter, Index 9 (97% over 49bp)
AGAGCGGAATATCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG19480.11274511789909775TruSeq Adapter, Index 9 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCA351.4429679E-744.0142441
TCCTGTG254.4352906E-544.0142441
ACACCGG700.044.0142441
GAATCGG454.783942E-1044.0142441
TGAACGG900.044.0142441
TTATCGG3000.044.0142441
AAAACGG207.8450673E-444.0142441
AGTGCTA454.783942E-1044.0142441
TAACCGG207.8450673E-444.0142441
TCAGCGG408.292773E-944.0142441
TCGCTCT207.8450673E-444.0142441
ACAGCGG1950.044.0142441
GTATCGG850.044.0142441
ATAACGG800.044.0142441
GGACCGG551.8189894E-1244.0142441
CTGGCCC207.8450673E-444.0142441
GCATCGG254.4352906E-544.0142441
CGAAACT650.044.014241
TGACCGG2400.044.014241
GGCTGGT302.5228346E-644.014241
Produced by FastQC (version 0.11.5)