FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005488435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6762795
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA270908840.058703538995346Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA3353694.959029513684801Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC1988612.9405149793835244Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC1188751.7577791430909853Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1187901.7565222663114881Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1041961.5407239166646336Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC784161.159520582835943Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC696131.0293525088369528Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG669280.9896499893904812Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA628850.9298670150433364Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA584130.8637405096561407Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC557940.8250139180619848Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG547330.809325138496731Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC540110.7986490792638251Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA390950.5780893846405222Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA340750.5038597207219796Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC298760.44177000781481623Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC283560.41929409364027737Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC272100.4023484372955265Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA267830.396034479826758Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA267420.39542822161547114Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC249860.3694626260296224Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC204460.3023306192188289Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA197820.2925121935531093Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG182520.2698884115221591Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG179910.2660290604698205No Hit
TAATCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA147730.21844518427661935Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC136020.20112985829083982Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA123500.1826168026681276Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA116920.1728870977162549Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACACGTCTGAACTC114100.1687172241654523Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC111530.1649170202556783Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACACGTCTGAACT110990.16411853383105654Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA109060.16126468420231574Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCC108790.16086544099000488Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CCCATAAAGTAGAAAGCACTAGATCGGAAGAGCACACGTCTGAACTCCAG103020.1523334656750648Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG102880.15212645067608882TruSeq Adapter, Index 20 (97% over 49bp)
AAACCGTTACCATTACTAAGATCGGAAGAGCACACGTCTGAACTCCAGTC102210.15113573603813216Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC102140.15103222853864418Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCCGTC98140.14511751428218658TruSeq Adapter, Index 20 (98% over 50bp)
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACGTCTGAACTCC96430.14258897393755096Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACGTCTGAACTCC90640.13402742505132864Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGTCTGAACTCC90360.1336133950533766Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACCGAGTAGATCGGAAGAGCACACGTCTGAACTCCA89680.13260789362977882Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA84600.12509620652407769Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACGTCTGAACTCC84160.12444558795586735Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA83910.12407591831483876Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTAAACTCCA79560.11764366656094115Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA79390.11739229120504169Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC78550.1161502012111856Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC78190.11561787692810443Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCGTTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA76030.11242393122961734Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGACACGATCACTCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG75270.1113001355208904Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGCTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA70730.10458693483981106Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA70610.10440949341211732Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC69840.10327091091774925Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG69360.10256114520697435Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA69170.10228019627979261Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCA68830.1017774455679937Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)