FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7276917
Sequences flagged as poor quality0
Sequence length40
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGT86061511.826643068761125No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGT5052396.943036453487101No Hit
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACG4887076.715852331420023No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA4180985.74553756762651No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACAC2350913.23064011861067No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACG1823882.5063910994175145No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGT1645662.261479689819191No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACAC1633192.244343311872322No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACAC1224861.6832128221333293No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTC1201921.6516884829111007No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACG1175401.6152444778468684No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGA811341.1149501911317665No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGA739591.0163507430413181No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACG626060.8603368706830103No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACG576040.7915989697285266No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGT536260.7369329621321777No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAA531940.7309963821217145No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTC444840.6113028360774212No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGC432140.593850390213328TruSeq Adapter, Index 18 (100% over 39bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACAC376780.5177742167459104No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGG374860.5151357367412601No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGA361880.4972985125431553No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACG350420.48155008501539864No Hit
TCGCTCTGATACCAAATTGATGAGATCGGAAGAGCACACG335970.46169277456373353No Hit
TCCTGTACTGAGCTGCCCCGAGCAGATCGGAAGAGCACAC319340.43883968994012157No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAA295470.40603733696564076No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGT291310.4003206302888984No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACG278800.3831292840085987No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACG236560.3250827239062916No Hit
GATGGATATGTCTTCAAGGACAGATCGGAAGAGCACACGT228730.31432267263732705No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGAT219620.30180363469859556No Hit
TCCTGTACTGAGCTGCCCCGTAGATCGGAAGAGCACACGT213400.2932560588501971No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG211050.290026669261172No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAG208030.2858765600871908No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGA204230.2806545684113203No Hit
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGT194900.2678332046387227No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACG192570.26463129921641265No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAA186010.25561649253385743No Hit
TTGAATTGAAGTGCTTGAATTAGATCGGAAGAGCACACGT179030.2460245183502849No Hit
TATTGCACTTGTCCCGGCCTGAGATCGGAAGAGCACACGT171890.23621267083299152No Hit
TCCTGTACTGAGCTGCCCCTAGATCGGAAGAGCACACGTC164310.22579617164796575No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACAC156730.21537967246294001No Hit
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGT155580.21379933287682132No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGC148130.20356148077544378No Hit
TCCTGTACTGAGCTGCCCCGATAGATCGGAAGAGCACACG147470.20265450327384524No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTG146580.20143145785502295Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAG142020.1951650678439784No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCAC137350.18874751491600084No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGA137300.18867880449921307No Hit
TGACATGGGACTGCCTAAGCTAAGATCGGAAGAGCACACG133430.18336061823983976No Hit
CAACGGAATCCCAAAAGCAGCAAGATCGGAAGAGCACACG132140.1815878894867153No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGA129160.1774927486461643No Hit
CAACGGAATCCCAAAAGCAGCCAGATCGGAAGAGCACACG128060.1759811194768334No Hit
CAACGGAATCCCACAAGCAGCTAGATCGGAAGAGCACACG125640.17265553530430539No Hit
CTTCTCACTACTGCACTTGACTAGTCAGATCGGAAGAGCA122040.16770838529558602No Hit
TGATCTCTTCGTACTCTTCTTGAGATCGGAAGAGCACACG118190.16241768320292782No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACA116360.1599028819484955No Hit
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCAC115030.15807518486194086No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGT114500.1573468544439905No Hit
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACG113080.15539547860721786No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGT112980.15525805777364232No Hit
TGGAGAGAAAGGCAGTTCCTGAAGATCGGAAGAGCACACG109670.15070942818229202No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACA108850.14958257734697264No Hit
TCCTGTACTGAGCTGCCCCGGAGATCGGAAGAGCACACGT107120.14720519692611583No Hit
TCCTGTACTGAGCTGCCCCGAGGAGATCGGAAGAGCACAC107020.1470677760925403No Hit
GATGGATATGTCTTCAAGGATAGATCGGAAGAGCACACGT105200.1445667169214655No Hit
AAGCTGCCAGTTGAAGAACTAGATCGGAAGAGCACACGTC98260.1350297110713232No Hit
AGCTCGGTCTGAGGCCCCTCAAGATCGGAAGAGCACACGT96190.13218509981630958No Hit
GGCTGGTCCGAAGGTAGTAGATCGGAAGAGCACACGTCTG92700.1273891127245233Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGT92640.12730666022437798No Hit
TGTTTGTTGTACTCGGTCTAGTAGATCGGAAGAGCACACG91630.12591870980526507No Hit
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGT89150.12251067313259173No Hit
TCGCTCTGATACCAAATTGATAAGATCGGAAGAGCACACG86420.11875908437597957No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTC84400.11598318353775369No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACG79900.1097992460268545No Hit
AGATCGGAAGAGCCCACGTCTGAACTCCAGTCACGTCCGC77820.10694089268848332TruSeq Adapter, Index 18 (97% over 39bp)
CAACGGAATCCCCAAAGCAGCTAGATCGGAAGAGCACACG77430.10640495143753873No Hit
ATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT77100.10595146268673945No Hit
CCCCCCACTGCTAAATTTGACTGGTAGATCGGAAGAGCAC76320.10487958018485027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGT3550.034.0063321
CGAAACG200.002787413534.0063321
CGACGGA1000.034.0063321
CATTGCG650.034.0063321
TAGCGCT200.002787413534.0063321
TAAACTA2000.034.0063321
GGATCTC252.0269632E-434.0063321
CGACATG408.0626705E-834.0063321
TAACGGA750.034.0063321
AAACGGT200.002787413534.0063321
AAACCGA252.0269632E-434.0063321
TCGAATC3550.034.0063321
AATCCGT455.984475E-934.006331
TACCAAC950.034.006331
CAAAACG700.034.006331
CAACGGG351.0908225E-634.006331
CTATACG4100.034.006331
GTCTACG3400.034.006331
AAGCCGT5000.034.006331
TCATGTA351.0908225E-634.006331
Produced by FastQC (version 0.11.5)