FastQCFastQC Report
Fri 31 May 2024
EGAF00005488983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160944
Total Bases319.2 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA149820.4739723323159158No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG56060.17735208216279694No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT50650.1602369418755916No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG48010.15188500650438602No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA43840.13869274495214087No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC41830.13233388506724572No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG40380.12774664783684872No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC39820.1259750251823506No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC35240.11148568275806214No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA33620.10636063150754964No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT33420.10572790913094315No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA31940.1010457635440552No Hit
GGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAATAACA31610.1000017716226545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)