FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3183344
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT71740.22536050140983824No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC51950.16319317045220372No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG48610.15270105901215827No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA43640.13708854588131222No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC42380.13313044396081603No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT41500.13036605531792983No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA38200.11999959790710649No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC37930.11915143320985731No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT37400.11748651732266446No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA36810.11563312039163849No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC36590.11494202323091693No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA36110.1134341748802517No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA33400.10492111440045437No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT32550.10225096627948473No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT32200.10115149352379134No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC32180.10108866650918029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATA10650.045.042999
CCAGTAG11250.042.8607525
GTAGCAT11700.041.4092258
TCACCCG20400.040.959423
GCCAGTA12450.036.816954
CAGTAGC12950.036.6849826
CACCCGG23450.035.4284674
CTCACCC27100.030.8329262
ACCCGGC26500.030.8064165
TGCCAGT16100.028.7668153
CGGCCCG29700.027.6676628
CGCTACG6250.026.7416951
CCTCACC33200.025.602531
GTCTAAC5850.025.3050271
ATAGCTC18400.024.51245738-39
CGGACAG18550.024.31462918-19
CTCGATT18750.024.20188148-49
CGGACAC19000.024.1232612-13
ATTCCGT18350.024.07808952-53
ACGGACA18100.023.21414218-19
Produced by FastQC (version 0.11.5)