FastQCFastQC Report
Fri 31 May 2024
EGAF00005488989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330985
Total Bases134.4 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG93270.7007592121624211No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA92420.6943729643835205No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT78280.5881358542733389No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA68240.5127029981555014No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC65980.4957230922963069No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT59630.44801406477157896No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG55160.4144299146872429No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT53430.40143202214900997No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT52700.3959473622918365No Hit
CTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGA52600.3951960390237305No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA49140.3692002539472646No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG46490.34929018734245687No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC46100.3463600265968437No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA45800.3441060567925258No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC42220.31720868379433276No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC39210.29459385342434363No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA38800.2915134280251092No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC38690.2906869724301927No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC37130.2789663294477398No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT36040.270776905825385No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC35300.2652171136414009No Hit
GTCCTTTTTAGTTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT34460.2589059981893109No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTG33940.25499911719516No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC32790.24635889961194152No Hit
GGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTA31970.24019804881347273No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCC31830.2391461962381244No Hit
GGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAATAACA30340.22795147954334571No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA30180.2267493623143762No Hit
GTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCAACACGCTGTTGCT28300.2126244848739843No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC28020.21052077972328764No Hit
ATCCATGTCAACAGCCAAGCCAACACGCTGTTGCTCCAATACATAAAAGG26800.20135463585239502No Hit
CTTCCATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGT26020.19549431436116863No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC25730.19331547688366135No Hit
GCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACAT24000.18031758434542838No Hit
CTCCAATATGTAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT23890.17949112875051185No Hit
CGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTT23840.17911546711645887No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTG23470.17633557102446684No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCC23290.17498318914187613No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT23260.17475779216144433No Hit
CCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT23150.1739313365665278No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT23010.17287948399117947No Hit
AGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTG22720.1707006465136722No Hit
GTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTGCTGAGCC22600.16979905859194508No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG22550.1694233969578921No Hit
GTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTTCATCCATGTCAA22100.1660424422514153No Hit
GTCTTCTTCCACTTCTTGGTACTTTTCAATTTCATCCATGTCAACAGCCA21600.16228582591088556No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCC21410.16085831170148424No Hit
CTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGG21400.16078317937467365No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC21240.1595810621457041No Hit
GTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAA20700.15552391649793199No Hit
CTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTGCTCAGTTACCTGG20540.15432179926896247No Hit
CTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCA20100.15101597688929627No Hit
CTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAAGATCT19900.14951333035308437No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGG19810.148837139411789No Hit
CAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGC18890.14192496534521426No Hit
CCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCC18850.14162443603797187No Hit
CTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCTGTTTCCCAG18840.14154930371116128No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG18740.14079798044305533No Hit
CACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAGGA18510.13906993692641165No Hit
CCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGC17970.13501279127863952No Hit
GTCCTTTTTAATTCCTGCAATACATTCAGACAGGGACAGACAAAATAAGC17940.13478739429820774No Hit
CCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCA17770.1335101447424276No Hit
GGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTATCCAGTGAGTC17470.13125617493810973No Hit
GGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAAC17460.13118104261129915No Hit
CTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAAT16980.12757469092439058No Hit
GGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACA16920.12712389696352702No Hit
GGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCTGTTTCCCAGCA16850.12659797067585285No Hit
CCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGG16590.12464453017877737No Hit
GTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTG16590.12464453017877737No Hit
GTCGAATAACATCTATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTC16430.12344241294980784No Hit
GCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGGTCCTTTTTA16350.12284135433532309No Hit
GTCTTACCAGTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTAAGACG16250.12209003106721714No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG16010.12028685522376287No Hit
CCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAG15860.11915987032160393No Hit
CAGCTGTTTCCCAGCAAAGATCAACCTCTGCTGGTCAGGAGGGATGCCTT15860.11915987032160393No Hit
GTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCTGTTTCCCAGCAAAGA15340.11525298932745297No Hit
GTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGCTGAGCC15090.1133746811571881No Hit
CCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAG15000.11269849021589273No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG14970.11247309323546095No Hit
CCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCT14500.10894187387536299No Hit
GCCCGCTCTGTTTTCCCTGAACCAGGAGTCTCCAGATGTCAACACAGAAG14430.10841594758768881No Hit
GTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCAACACACTGTTGCT14390.10811541828044645No Hit
AGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAA14350.10781488897320406No Hit
CCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAA14260.10713869803190869No Hit
GTAGGGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAAT14220.10683816872466632No Hit
CCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTT14060.1056360514956968No Hit
CTCTTTCTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCTGTT14020.1053355221884544No Hit
GGGCATTTTCACCTTGGGCATCTTCAGGTGCCAGTCTGGGCCATGAACAT13980.10503499288121204No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGTTTTGATCTTCTTCCCCTTCTTTT13930.10465933124715907No Hit
GGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTTC13890.10435880193991667No Hit
CTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAGGAGT13750.10330694936456836No Hit
GCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAGG13500.10142864119430346No Hit
CAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGC13490.10135350886749288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)