FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005488991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005488991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307799
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA133840.40461950680800135No Hit
GGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGT85680.25902420310303015No Hit
GGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCT80770.24418049585237794No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG51640.15611589458730715No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG49260.1489207778344452No Hit
GGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTG49120.14849753567251214No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG44700.13513517598862568No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG43840.1325352598510369No Hit
GGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTC41460.12534014309817496No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC40800.12334485862049054No Hit
CAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCG40380.12207513213469137No Hit
AGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTG38730.11708692094048036No Hit
CCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAG38460.11627066819960948No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT36870.11146384650336975No Hit
GCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTA36470.11025458318356103No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT34610.10463150874645043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC30400.043.275574
CAGACCG30300.043.261653
ACCGCGT30600.043.147936
CCGCGTT31000.042.5911837
CGCGTTC31750.041.585098
GACCGCG32150.040.919985
GCGTTCT33550.039.3549
TCAGACC33400.038.8197562
CTCAGAC40300.035.0222551
TACGGAG30100.032.34918634-35
AATACGG30350.032.08271832-33
AGAACGA32100.030.19162644-45
ACACGCG3150.030.1547728
GTCCGTT1452.3923349E-829.4962751
CGATCAT33200.029.4712548-49
TGACGGC33450.028.9685362-63
AAGCCGG33550.028.6676592-93
AACGATC35750.027.23933246-47
AGCAACG35900.027.12367280-81
CAACGCC36150.027.00138782-83
Produced by FastQC (version 0.11.5)